NIDOGEN MEDIATES THE FORMATION OF TERNARY COMPLEXES OF BASEMENT-MEMBRANE COMPONENTS
NIDOGEN MEDIATES THE FORMATION OF TERNARY COMPLEXES OF BASEMENT-MEMBRANE COMPONENTS
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DOI：
10.1038/ki.1993.3
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发表时间：
1993-01-01
影响因子：
19.6
通讯作者：
FOX, JW
中科院分区：
文献类型：
--
作者：
AUMAILLEY, M;BATTAGLIA, C;FOX, JW
使用重组巢蛋白，我们探测了各种巢蛋白结构域的钙结合潜力，检查了巢蛋白与各种基底膜蛋白的结合，并评估了巢蛋白介导层粘连蛋白和硫酸乙酰肝素蛋白聚糖以及IV型胶原和层粘连蛋白之间三元复合物形成的能力。这些实验的结果表明，Ca+2 结合在杆状结构域上，并且在 N 端 G1 结构域上观察到额外的结合。关于巢蛋白在介导基底膜成分之间的复合物形成中的作用，已证明巢蛋白有效地促进层粘连蛋白和IV型胶原之间三元复合物的形成，这两种成分独立地与巢蛋白相互作用。类似地，巢蛋白介导层粘连蛋白和蛋白聚糖之间的三元复合物形成。有趣的是，蛋白多糖和巢蛋白之间的相互作用是通过蛋白多糖的蛋白质核心进行的。我们将 nidogen 与蛋白聚糖核心和 IV 型胶原的主要相互作用位点定位于球状 G2 结构域上的区域，而 C 端球状 G3 与层粘连蛋白结合。 Ca+2 结合在二元或三元复合物形成中似乎并不重要。报告的数据使我们能够假设，通过巢蛋白与其他基底膜成分的多重相互作用，巢蛋白在基底膜组织和稳定中发挥着至关重要的结构作用。
Using a recombinant nidogen we have probed the calcium binding potential of various nidogen domains, examined the binding of nidogen to various basement membrane proteins and assessed the ability of nidogen to mediate the formation of ternary complexes between laminin and heparan sulfate proteoglycan and collagen IV and laminin. The results of these experiments indicate that the Ca+2 binding is on the rod-like domain with additional binding observed on the N-terminal G1 domain. With regard to the role of nidogen in mediating complex formation among basement membrane components it was demonstrated that nidogen effectively promotes the formation of a ternary complex between laminin and collagen IV, with both of these components interacting independently with nidogen. Similarly, nidogen mediates a ternary complex formation between laminin and proteoglycan. Interestingly, the interaction between proteoglycan and nidogen is through the protein core of the proteoglycan. We have localized the major interaction sites on nidogen with the proteoglycan core and collagen IV to a region on the globular G2 domain while the C-terminal globe G3 binds to laminin. Ca+2 binding does not appear to be important in either of the binary or ternary complex formations. The data reported allow us to hypothesize that, via the multiple interactions of nidogen with other basement membrane components, nidogen plays a crucial structural role in basement membrane organization and stabilization.