Inhibition Of Tumorigenesis in ApcMin/+ Mice by a Combination of (-)-Epigallocatechin-3-gallate and Fish Oil
Inhibition Of Tumorigenesis in ApcMin/+ Mice by a Combination of (-)-Epigallocatechin-3-gallate and Fish Oil
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DOI：
10.1021/jf071004r
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发表时间：
2007-09-19
影响因子：
6.1
通讯作者：
Yang, Chung S.
中科院分区：
文献类型：
--
作者：
Bose, Mousumi;Hao, Xingpei;Yang, Chung S.
本研究研究了(-)-表没食子儿茶素-3-没食子酸酯（EGCG）与鱼油联合使用对高脂饲料喂养的Apc（Min/+）小鼠肠道肿瘤发生的影响。与对照组相比，EGCG和鱼油联合治疗9周，肿瘤数量减少了53%，而单独使用两种药物都没有显著效果。各治疗组细胞凋亡均显著增加。联合用药组腺瘤β - catenin核阳性低于对照组，提示联合用药对Wnt信号通路的调节作用。与对照组相比，鱼油和联合用药显著降低了小肠肿瘤中前列腺素E-2 （PGE(2)）的水平，表明鱼油调节了花生四烯酸的异常代谢。在所有治疗组中，腺瘤中Akt磷酸化显著降低，这可能是观察到的细胞凋亡增加的原因。结果表明，低剂量EGCG和鱼油联合使用可抑制Apc（Min/+）小鼠肿瘤的多样性。
The effect of a combination of (-)-epigallocatechin-3-gallate (EGCG) with fish oil on intestinal tumorigenesis in Apc(Min/+) mice fed a high-fat diet was investigated in the present study. The combined treatment of EGCG and fish oil for 9 weeks reduced the tumor number by 53% as compared to controls while neither agent alone had a significant effect. Apoptosis was significantly increased in all treatment groups. beta-Catenin nuclear positivity in adenomas from the combination group was lower than control mice, implicating the modulation of Wnt signaling by the combination. Fish oil and the combination significantly reduced prostaglandin E-2 (PGE(2)) levels in small intestinal tumors as compared to controls, suggesting modulation of aberrant arachidonic acid metabolism by fish oil. Akt phosphorylation in adenomas was significantly reduced in all treatment groups, which may have contributed to the observed increase in apoptosis. The results indicate that a combination of low doses of EGCG and fish oil can inhibit tumor multiplicity in Apc(Min/+) mice.