Consecutive treatments with photodynamic therapy and nystatin altered the expression of virulence and ergosterol biosynthesis genes of a fluconazole-resistant Candida albicans in vivo
Consecutive treatments with photodynamic therapy and nystatin altered the expression of virulence and ergosterol biosynthesis genes of a fluconazole-resistant Candida albicans in vivo
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DOI：
10.1016/j.pdpdt.2020.102155
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发表时间：
2021-01-23
影响因子：
3.3
通讯作者：
Pavarina, Ana Claudia
中科院分区：
文献类型：
--
作者：
Jordao, Claudia Carolina;Klein, Marlise Inez;Pavarina, Ana Claudia
本研究评估了连续五天局部治疗的抗菌光动力疗法（aPDT），制霉菌素（NYS），和治疗的氟康唑耐药菌株的白色念珠菌定殖小鼠的舌头上的协会的效果。最后一次处理后，C.从舌中回收白色念珠菌并用于测定它们对氟康唑的敏感性。最后一次治疗24小时后，处理小鼠舌以评估C.与白念珠菌毒力和麦角固醇产生有关的基因。氟康唑药敏试验显示所有治疗组和对照组（未治疗）的耐药特征相似。aPDT、NYS、NYS+aPDT和aPDT+NYS处理促进了ALS 1、EFG 1、CAP 1、SOD 1、SAP 1和LIP 3表达的降低。HWP 1的表达在三个含制霉菌素的组中均较高。相反，治疗产生了CAT1基因表达的显着增加，主要是在其中进行aPDT的组中。与麦角固醇产生相关的基因的表达通过所评价的处理（aPDT、NYS、NYS+aPDT和aPDT+NYS）显著降低。因此，对小鼠舌头进行的连续局部治疗促进了氟康唑耐药C.白色念珠菌
This investigation assessed the effect of five consecutive daily topical treatments of antimicrobial photodynamic therapy (aPDT), nystatin (NYS), and an association of treatments on a fluconazole-resistant strain of Candida albicans colonizing the tongues of mice. After the last treatments application, colonies of C. albicans were recovered from the tongues and used to determine their fluconazole susceptibility. After 24 hours of the last treatment, the mice tongues were processed to evaluate the expression of C. albicans genes related to the viru-lence and ergosterol production. The fluconazole susceptibility test yielded a resistance profile similar for all treatment groups and the control group (no treatment). The treatments aPDT, NYS, NYS+aPDT, and aPDT+NYS promoted a reduction in ALS1, EFG1, CAP1, SOD1, SAP1, and LIP3 expression. The expression of HWP1 was higher in the three groups containing nystatin. In contrast, the treatments produced a significative increase in CAT1 gene expression, mainly in the groups in which aPDT was performed. The expression of genes related to ergosterol production was significantly reduced by the treatments evaluated (aPDT, NYS, NYS+aPDT, and aPDT+NYS). Thus, the consecutive topical treatments performed on mice tongues promoted a reduction in the expression of virulence and ergosterol biosynthesis genes of a fluconazole-resistant C. albicans.