Non-destructive DNA extraction for small pelagic copepods to perform integrative taxonomy
Non-destructive DNA extraction for small pelagic copepods to perform integrative taxonomy
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DOI：
10.1093/plankt/fbu105
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发表时间：
2015-01-01
影响因子：
2.1
通讯作者：
Cornils, Astrid
中科院分区：
文献类型：
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作者：
Cornils, Astrid
对小型桡足类的分子分析（千分之一毫米）通常涉及标本的完全破坏。因此，分子和形态学结果之间的不一致性无法调查，因为没有标本凭证。本研究提供了一种改进的基于柱的DNA提取方法，以保留标本的外骨骼，从而使相同标本的分子和形态学分析。该方法已被测试的九个浮游桡足类属的乙醇保存标本。
Molecular analyses of small-sized copepods (a parts per thousand currency sign1 mm) generally involve the complete destruction of the specimens. Consequently, incongruences between the molecular and morphological results cannot be investigated since no specimen vouchers remain. The present study provides a modified column-based DNA extraction method to retain the exoskeleton of the specimen and thus, to enable molecular and morphological analysis of the same specimens. The method has been tested on ethanol preserved specimens of nine pelagic copepod genera.