A functional AMPA receptor-calcium channel complex in the postsynaptic membrane
A functional AMPA receptor-calcium channel complex in the postsynaptic membrane
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DOI：
10.1073/pnas.0601289103
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发表时间：
2006-04-04
影响因子：
11.1
通讯作者：
Campbell, KP
中科院分区：
文献类型：
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作者：
Kang, MG;Chen, CC;Campbell, KP
Ca~(2+)通道在突触活动的调节中起着关键作用。与突触前膜上电压激活的钙通道释放神经递质的功能相比，一些研究才刚刚开始阐明突触后膜上的钙通道的功能。在这项研究中，我们证明了alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate受体与神经元钙通道的功能联系。一系列生化研究表明，Ca(V)2.11(α(1A)类)和Ca(V)2.2(α(1B)类)与突触后膜上的AMPA受体具有特异性联系。我们对重组Ca(V)2.1和AMPA受体的电生理和钙成像分析也表明这两个通道具有功能偶联。考虑到突触后细胞内钙离子浓度([Ca~(2+)](I))升高和AMPA受体转运在长时程增强(LTP)和长时程抑制(LTD)中的重要作用，钙通道与AMPA受体的功能联系可能为突触可塑性的机制提供新的见解。
Ca2+ channels play critical roles in the regulation of synaptic activity. In contrast to the well established function of voltage-activated Ca2+ channels in the presynaptic membrane for neurotransmitter release, some studies are just beginning to elucidate the functions of the Ca2+ channels in the postsynaptic membrane. In this study, we demonstrated the functional association of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) receptors with the neuronal Ca2+ channels. A series of biochemical studies showed the specific association of Ca(v)2.11 (alpha(1A)-class) and Ca(v)2.2 (alpha(1B)-class) with AMPA receptors in the postsynaptic membrane. Our electrophysiological and Ca2+ imaging analyses of recombinant Ca(v)2.1 and AMPA receptors also showed functional coupling of the two channels. Considering the critical roles of postsynaptic intracellular concentration of Ca2+ ([Ca2+](i)) increase and AMPA receptor trafficking for long-term potentiation (LTP) and long-term depression (LTD), the functional association of Ca2+ channels with the AMPA receptors may provide new insights into the mechanism of synaptic plasticity.