Induction of intestinal glucose carriers in streptozocin-treated chronically diabetic rats.
Induction of intestinal glucose carriers in streptozocin-treated chronically diabetic rats.
复制标题
链佐星治疗的慢性糖尿病大鼠肠道葡萄糖载体的诱导。
DOI：
10.1016/0016-5085(89)90761-0
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发表时间：
1989
期刊：
影响因子：
29.4
通讯作者：
Field,M
中科院分区：
文献类型：
--
作者：
Fedorak,RN;Gershon,MD;Field,M
在实验诱导的慢性糖尿病中，肠葡萄糖吸收的最大转运能力（Vmax）增加。用[~ 3 H]根皮苷放射自显影法研究了转运Vmax的增加与钠-葡萄糖共转运体在大鼠回肠腔表面的数量和分布之间的关系。雄性刘易斯大鼠用链脲佐菌素制成糖尿病。90天后，我们测量了3-O-甲基-D-吡喃葡萄糖的吸收和特定的[3 H]根皮苷结合到相同大鼠的回肠粘膜。与年龄匹配的对照组相比，糖尿病大鼠的净3-O-甲基-D-吡喃葡萄糖通量高6.9倍。[3 H]根皮苷的管腔表面的特异性结合是7.2倍以上的糖尿病大鼠。放射自显影显示，在慢性糖尿病患者中，特异性根皮苷结合延伸到回肠的中绒毛区域，而在年龄匹配的对照组中，它仅限于绒毛尖端。我们认为，在未经治疗的糖尿病，肠绒毛上皮细胞参与葡萄糖吸收的大部分。
The maximal transport capacity (Vmax) for intestinal glucose absorption is increased in experimentally induced chronic diabetes mellitus. Using [3H]phlorizin radioautography, we examined the relation between this increase in transport Vmaxand the number and distribution of sodium-glucose co-transporters on the luminal surface of rat ileum. Male Lewis rats were made diabetic with streptozocin. Ninety days later we measured 3-O-methyl-d-glucopyranose absorption and specific [3H]phlorizin binding to the ileal mucosa of the same rats. Net 3-O-methyl-d-glucopyranose flux was 6.9-fold greater in diabetic rats compared with age-matched controls. Specific binding of [3H]phlorizin to the luminal surface was 7.2-fold greater in the diabetic rats. Radioautography revealed that, in chronic diabetes, specific phlorizin binding extends into the midvillus region of the ileum, whereas in age-matched controls, it is confined to villus tips. We believe that, in untreated diabetes, a larger fraction of intestinal villus epithelial cells participate in glucose absorption.