Methylation Profile of BRCA1, RASSF1A and ER in Vietnamese Women with Ovarian Cancer
Methylation Profile of BRCA1, RASSF1A and ER in Vietnamese Women with Ovarian Cancer
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DOI：
10.7314/apjcp.2013.14.12.7713
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发表时间：
2013-01-01
影响因子：
--
通讯作者：
Ta Van To
中科院分区：
文献类型：
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作者：
Vo Thi Thuong Lan;Ta Bich Thuan;Ta Van To
DNA甲基化被认为是一种有前途的生物标志物，用于诊断癌症，特别是卵巢癌。在我们的研究中，我们验证了甲基化特异性聚合酶链反应（MSP）的准确性，分析BRCA 1，RASSF 1A和ER的甲基化模式在59和10例越南上皮性卵巢癌（EOC）和良性卵巢肿瘤，分别。BRCA 1、RASSF 1A和ER基因甲基化分别在11/59（18.6%）、40/59（67.8%）和15/59（25.4%）的EOC患者中检出，而BRCA 1基因甲基化仅在2/10（20%）的良性卵巢疾病患者中检出。59个EOC中有45个（78%）在一个或多个基因上表现出甲基化。RASSF 1A基因甲基化频率与卵巢上皮性癌显著相关（p
DNA methylation is considered a promising biomarkers for diagnosis of cancer in general and of ovarian cancer in particular. In our study, we validated the accuracy of methylation specific polymerase chain reaction (MSP) to analyze the methylation pattern of BRCA1, RASSF1A and ER in 59 and 10 Vietnamese patients with epithelial ovarian cancer (EOC) and benign ovarian tumors, respectively. We found methylation of BRCA1, RASSF1A and ER in 11/59 (18.6%), 40/59 (67.8%) and 15/59 (25.4%) of EOC cases, while methylation of BRCA1 was only detected in 2/10 (20%) benign ovarian patients. Forty five out of the 59 EOCs (78%) demonstrated methylation at one or more genes. The methylation frequency of RASSF1A was significantly associated with EOC (p