Concordance between antioxidant activities in vitro and chemical components of Radix Astragali (Huangqi)
Concordance between antioxidant activities in vitro and chemical components of Radix Astragali (Huangqi)
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DOI：
10.1080/14786419.2010.551771
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发表时间：
2012-01-01
影响因子：
2.2
通讯作者：
Wang, Xiaozhen
中科院分区：
文献类型：
--
作者：
Li, Xican;Chen, Dongfeng;Wang, Xiaozhen
采用ABTS法、DPPH中心点法、还原力法、O-中心点（2）法和（OH）-O-中心点法测定了5种黄芪提取物的体外抗氧化活性。测定其总酚、总黄酮、总皂苷和总糖的含量。使用各种抗氧化测定的1/IC 50值来评价RA提取物的抗氧化活性水平（显示1/IC 50值与化学含量的图），并且总酚和总黄酮的平均R值（相关系数）为0.762和0.638。总皂苷和总糖的平均R值分别为-0.0386和-0.132。这种巨大的差异清楚地表明，RA在体外的抗氧化作用通常可能被认为是酚类化合物（包括黄酮类化合物）的存在的结果，而不是甘草苷和多糖。
Five Radix Astragali (RA) extracts were prepared and their antioxidant activities were measured in vitro using ABTS [2, 2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid diammonium salt)], DPPH center dot(1,1-diphenyl-2-picrylhydrazyl radical), reducing power, O-center dot(2)- and (OH)-O-center dot assays. Their chemical contents were then determined, including total phenolics, total flavonoids, total saponins and total sugars. The 1/IC50 values of the various antioxidant assays were used to evaluate the level of antioxidant activity of the RA extracts (plot showing 1/IC50 values vs. chemical contents), and the average R values (correlation coefficients) of total phenolics and total flavonoids were 0.762 and 0.638. In contrast, the average R values of the total saponins and total sugars were -0.0386 and -0.132, respectively. This large difference clearly demonstrates that the antioxidant effects of RA in vitro might be generally considered to be a result of the presence of phenolic compounds (including flavonoids) but not astragalosides and polysaccharides.