Diminished production of T helper 1 cytokines correlates with T cell unresponsiveness to Brucella cytoplasmic proteins in chronic human brucellosis
Diminished production of T helper 1 cytokines correlates with T cell unresponsiveness to Brucella cytoplasmic proteins in chronic human brucellosis
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DOI：
10.1086/341449
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发表时间：
2002-07-15
影响因子：
6.4
通讯作者：
Fossati, CA
中科院分区：
文献类型：
--
作者：
Giambartolomei, GH;Delpino, MV;Fossati, CA
本研究检测了25例布鲁氏菌病患者外周血单个核细胞（PBMC）抗布鲁氏菌胞浆蛋白（CP）的细胞免疫应答。体外增殖和细胞因子的基因表达和生产进行了研究。来自14名患者的PBMC对CP有反应而增殖（反应患者[RP]），而来自11名患者的细胞没有增殖（无反应患者[NRP]）。CP特异性白细胞介素（IL）-2和干扰素-γ显着诱导PBMC从RP，与细胞从NRP相比。两组间IL-10的产生无显著差异。CP不诱导IL-4的产生。观察到患者的临床状态与对CP的T细胞应答之间存在密切关系。急性感染患者对CP有反应，并诱导产生辅助性T细胞1（Th 1）细胞因子，而慢性感染患者则无反应。Th 1细胞因子的产生减少可能导致慢性人布鲁氏菌病T细胞无反应性。
This study evaluated the cellular immune response against Brucella species cytoplasmic protein (CP) in peripheral blood mononuclear cells (PBMC) of 25 patients with brucellosis. In vitro proliferation and cytokine gene expression and production were investigated. PBMC from 14 patients proliferated in response to CP (responder patients [RPs]) and cells from 11 patients did not (nonresponder patients [NRPs]). CP-specific interleukin (IL)-2 and interferon-gamma were significantly induced in PBMC from RPs, compared with cells from NRPs. No significant differences were found in the production of IL-10 between the 2 groups. CP did not induce IL-4 production. A close relationship was observed between the clinical status of the patients and the T cell response against CP. Patient with acute infections responded to CP and induced production of T helper 1 (Th1) cytokines, whereas chronically infected patients did not. Diminished production of Th1 cytokines may contribute to T cell unresponsiveness in chronic human brucellosis.