A major zebrafish polymorphism resource for genetic mapping
A major zebrafish polymorphism resource for genetic mapping
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DOI：
10.1186/gb-2007-8-4-r55
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发表时间：
2007-01-01
期刊：
影响因子：
12.3
通讯作者：
Smith, Jeffrey R.
中科院分区：
文献类型：
--
作者：
Bradley, Kevin M.;Elmore, J. Bradford;Smith, Jeffrey R.
我们在斑马鱼中鉴定了645，088个候选多态性，观察到单核苷酸多态性（SNP）验证率为71%至86%，随着多态性置信度评分的提高而提高。变异位点是非随机的，具有过量的特异性新颖的富含T和A的基序。我们将一半的多态性定位在斑马鱼的遗传和物理图谱上，作为定位克隆的资源。我们进一步证明了使用锚定SNPs作为斑马鱼高通量遗传作图方法的散装分离分析。
We have identified 645,088 candidate polymorphisms in zebrafish and observe a single nucleotide polymorphism ( SNP) validation rate of 71% to 86%, improving with polymorphism confidence score. Variant sites are non-random, with an excess of specific novel T- and A-rich motifs. We positioned half of the polymorphisms on zebrafish genetic and physical maps as a resource for positional cloning. We further demonstrate bulked segregant analysis using the anchored SNPs as a method for high-throughput genetic mapping in zebrafish.