Expression and function of endothelial selectins during human development
Expression and function of endothelial selectins during human development
复制标题
DOI：
10.1111/imm.12318
复制
发表时间：
2014-11-01
期刊：
影响因子：
6.4
通讯作者：
Salmi, Marko
中科院分区：
文献类型：
--
作者：
Auvinen, Kaisa;Jalkanen, Sirpa;Salmi, Marko
白细胞运输对于免疫防御至关重要。在成人中，白细胞和内皮细胞之间的早期束缚和滚动相互作用由P-、E-和L-选择素及其配体介导。相比之下，选择素在人类胎儿发育过程中单核细胞迁移中的作用仍然未知。我们研究了内皮细胞E-和P-选择素及其反受体在人类个体发育过程中的功能。免疫组织化学染色显示，P-选择素分别从妊娠第7周和第11周开始在巨核细胞和内皮细胞中表达。内皮E-选择素最晚出现在第32周。使用体外流动室测定的实时成像显示，脐带血单核白细胞使用E-、P-和L-选择素和PSGL-1在生理剪切应力下滚动并粘附于内皮。这些数据表明，选择素在人类出生前就已合成并发挥功能，并有可能介导单核细胞的迁移和炎症反应。
Leucocyte trafficking is vital for the immune defence. In adults, early tethering and rolling interactions between leucocytes and endothelial cells are mediated by P-, E- and L-selectins and their ligands. In contrast, the role of selectins in migration of mononuclear cells during fetal development in humans remains unknown. We studied the functions of endothelial E- and P- selectins and their counter-receptors during human ontogeny. Immunohistochemical stainings showed that P- selectin is expressed in megakaryocytes and endothelial cells starting from gestational weeks 7 and 11, respectively. Endothelial E- selectin appeared latest, at week 32. Real-time imaging using in vitro flow chamber assays showed that cord blood mononuclear leucocytes used E-, P- and L-selectin and PSGL-1 to roll on and adhere to endothelium under physiological shear stress. These data show that selectins are synthesized and functional before birth in humans and have the potential to mediate the emigration of mononuclear cells and inflammatory responses.