The intra-axonal transport of acetylcholine and cholinergic enzymes in rat sciatic nerve during regeneration after various types of axonal trauma.
The intra-axonal transport of acetylcholine and cholinergic enzymes in rat sciatic nerve during regeneration after various types of axonal trauma.
复制标题
各种类型轴突创伤后再生过程中大鼠坐骨神经乙酰胆碱和胆碱能酶的轴突内转运。
DOI：
10.1002/neu.480100203
复制
发表时间：
1979
期刊：
影响因子：
--
通讯作者：
S. Bööj
中科院分区：
文献类型：
--
作者：
P. ;A. Dahlström;P. Larsson;S. Bööj
用累积法研究了大鼠再生坐骨神经轴突内乙酰胆碱（ACh）和胆碱能酶（胆碱乙酰转移酶（CAT）和乙酰胆碱酯酶（AChE））的近端-远端转运。采用四种类型的轴索损伤：用固体CO2冷冻、挤压、用剩余的紧丝线结扎神经和切断神经。正常和假手术大鼠作为对照。1 ~ 29天后，在创伤近端约15 mm处压碎神经。12小时后，解剖出近端的神经节段，测定乙酰胆碱含量和酶活性。破碎后12小时该段的增加被视为再生神经中近端-远端运输的指示。乙酰胆碱运输似乎没有变化，在再生过程中相比，控制。与此相反，CAT和AChE的运输最初显着抑制。在观察期（29天）结束时，所有组的CAT转运均恢复。在冷冻和挤压组中，AChE转运的恢复是显著的。在切割组中未观察到恢复，在结扎组中仅发生小的恢复。因此，在神经的再生促进了完整的结缔组织鞘（冷冻和破碎）的存在下，恢复运输发生早于在切割或结扎的神经。
The proximo-distal intra-axonal transport of acetylcholine (ACh) and cholinergic enzymes (choline acetyltransferase, CAT, and ACh-esterase, AChE) in rat regenerating sciatic nerve was studied by accumulation technique. Four types of axonal trauma were performed: freezing with solid CO2, crushing, ligating the nerve with remaining tight silk ligature, and cutting the nerve. Normal and sham-operated rats were used as controls. One to twenty-nine days later, the nerves were crushed about 15 mm proximal to the trauma. The nerve segment proximal to this crush was dissected out 12 hr later and assayed for ACh-content and enzyme activities. The increase in this segment 12 hr after crushing was taken as an indication of proximo-distal transport in the regenerating nerves. ACh transport did not seem to vary during regeneration as compared to controls. In contrast, the transport of both CAT and AChE was initially markedly depressed. Towards the end of the observation period (29 days), a recovery of CAT-transport occurred in all groups. Recovery of AChE-transport was marked in the freeze and crush groups. In the cut group no recovery was seen and in the ligated group only a small recovery occurred. Thus, in the nerves where regeneration was facilitated by the presence of intact connective tissue sheaths (freezing and crushing) recovery of transport occurred earlier than in cut or ligated nerves.