Regulatory B Cells Shape the Development of Th2 Immune Responses in BALB/c Mice Infected with Leishmania major through IL-10 Production
Regulatory B Cells Shape the Development of Th2 Immune Responses in BALB/c Mice Infected with Leishmania major through IL-10 Production
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DOI：
10.4049/jimmunol.0901114
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发表时间：
2010-01-15
影响因子：
4.4
通讯作者：
Launois, Pascal
中科院分区：
文献类型：
--
作者：
Ronet, Catherine;Torre, Yazmin Hauyon-La;Launois, Pascal
最近的证据表明，B细胞是需要与感染与利什曼原虫在BALB/c小鼠的易感性。在这项研究中，我们分析了由B细胞产生的IL-10在这一过程中的作用。结果表明，从BALB B/c小鼠脾中纯化的B细胞对L.主要是体外。在体内，L major感染后，在易感的BALB B/c小鼠的引流淋巴结的B细胞中检测到早期IL-10 mRNA表达，但在耐药的C57 BL/6小鼠中未检测到。虽然在B细胞缺陷型BALB/c小鼠中，在感染前过继转移初始野生型B细胞恢复了Th 2细胞发育和对L.在这些其他抗性小鼠中，过继转移IL-10（-/-）B细胞的小鼠没有。L.主要的，在体外或体内相遇后，表达CD 1d和CD 5分子，并且由这些细胞产生的IL-10下调L.主要刺激的树突状细胞。这些观察结果表明分泌IL-10的B细胞是表型和功能调节性B细胞。总之，这些结果表明，调节性CD 1d（+）CD 5（+）B细胞响应L. major对BALB/c小鼠Th 2细胞发育至关重要。免疫学杂志，2010，184：886-894。
Recent evidence indicates that B cells are required for susceptibility to infection with Leishmania major in BALB/c mice. In this study, we analyzed the role of the IL-10 produced by B cells in this process. We showed that B cells purified from the spleen of BALB/c mice produced IL-10 in response to stimulation with L. major in vitro. In vivo, early IL-10 mRNA expression is detected after L major infection in B cells from draining lymph nodes of susceptible BALB/c, but not of resistant C57BL/6 mice. Although adoptive transfer of naive wild-type B cells prior to infection in B cell-deficient BALB/c mice restored Th2 cell development and susceptibility to infection with L. major of these otherwise resistant mice, adoptive transfer of IL-10(-/-) B cells mice did not. B cells stimulated by L. major, following in vitro or in vivo encounter, express the CD1d and CD5 molecules and the IL-10 produced by these cells downregulate IL-12 production by L. major-stimulated dendritic cells. These observations indicate that IL-10 secreting B cells are phenotypically and functionally regulatory B cells. Altogether these results demonstrate that the IL-10 produced by regulatory CD1d(+) CD5(+) B cells in response to L. major is critical for Th2 cell development in BALB/c mice. The Journal of Immunology, 2010, 184: 886-894.