CONTINUOUS LABELING STUDIES ON MOUSE SKIN AND INTESTINE
CONTINUOUS LABELING STUDIES ON MOUSE SKIN AND INTESTINE
复制标题
DOI：
10.1111/j.1365-2184.1974.tb00907.x
复制
发表时间：
1974-01-01
期刊：
影响因子：
--
通讯作者：
HAMILTON, E
中科院分区：
文献类型：
--
作者：
POTTEN, CS;KOVACS, L;HAMILTON, E
数据显示了在重复（每 3 小时一次，共 168 小时）3H-TdR (5 μCi) 注射条件下小鼠空肠、结肠和背部皮肤这三种组织中绝对放射性水平和标记指数 (LI) 的变化。估计了 S 的长度和细胞周期。评估隐窝和粘膜通过时间。隐窝中有一个缓慢循环的亚群，可能是杯状细胞前体或隐窝干细胞群。皮肤 LI 数据不能用单条直线拟合，并且连续处理或注射动物的程序似乎会导致稳态的扰乱。所有样品的液体闪烁数据都显示出一些一致且令人惊讶的大波动，以及随着时间的增加与线性吸收的明显偏差。
Data are presented for changes in absolute radioactivity levels and labelling indices (LI) in three tissues: jejunum, colon and dorsal skin in the mouse during conditions of repeated (every 3 hr for 168 hr)3H‐TdR (5 μCi) injections. The lengths of S and the cell cycle were estimated. The crypt and mucosal transit times were evaluated. There is a slowly cycling subpopulation in the crypt which may be the goblet cell precursors or a crypt stem cell population. The skin LI data cannot be fitted by a single straight line and the procedure of continuously handling or injecting the animals appears to result in a disturbance of the steady state. The liquid scintillation data for all samples showed some consistent and surprisingly large fluctuations and a clear deviation from linear uptake with increasing time.