A high resolution atmospheric pressure matrix-assisted laser desorption/ionization-quadrupole-orbitrap MS platform enables in situ analysis of biomolecules by multi-mode ionization and acquisition
A high resolution atmospheric pressure matrix-assisted laser desorption/ionization-quadrupole-orbitrap MS platform enables in situ analysis of biomolecules by multi-mode ionization and acquisition
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DOI：
10.1016/j.aca.2017.12.045
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发表时间：
2018-05-12
影响因子：
6.2
通讯作者：
Li, Lingjun
中科院分区：
文献类型：
--
作者：
Chen, Bingming;OuYang, Chuanzi;Li, Lingjun
大气压（AP）/基质辅助激光解吸/电离（MALDI）于2000年推出，由于其易于样品引入和处理、与ESI源的可互换性以及分析挥发性物质的能力而引起质谱界的极大关注。在这项研究中，一个AP/MALDI源与超高空间分辨率耦合到Q Exactive HF轨道阱质谱仪的高分辨率原位分析MALDI，激光喷雾电离（LSI）和基质辅助电离（MAI）没有仪器修改。LSI和MAI产生多电荷离子，这扩大了质量检测范围并提高了裂解效率。对肽和蛋白质标准品、组织提取物和组织切片进行全MS、靶向MS/MS、数据依赖性采集（DDA）和平行反应监测（PRM）采集，以深入表征各种生物分子。高分辨率全MS和MS/MS图像从甲壳类动物和大鼠组织中获得，像素大小小于30 mm。总体而言，AP/MALDI-Q-Orbitrap是一种快速扫描仪器，能够执行多种类型的电离和多种采集模式，无需仪器修改。该仪器平台为其他高分辨率MALDI仪器提供了一种有吸引力的替代方案。(C)2018 Elsevier B. V.版权所有。
Introduced in 2000, atmospheric pressure (AP)/matrix-assisted laser desorption/ionization (MALDI) has attracted substantial attention in the mass spectrometry community due to its ease of sample introduction and handling, interchangeability with ESI source and capability of analyzing volatile species. In this study, an AP/MALDI source with ultra-high spatial resolution was coupled to a Q Exactive HF orbitrap mass spectrometer for high resolution in situ analysis by MALDI, laserspray ionization (LSI) and matrix assisted ionization (MAI) without instrument modification. LSI and MAI generated multiply charged ions, which expanded the mass detection range and improved fragmentation efficiency. Full MS, targeted MS/MS, data dependent acquisition (DDA) and parallel reaction monitoring (PRM) acquisitions were performed on peptide and protein standards, tissue extracts and tissue sections for in depth characterization of various biomolecules. High resolution full MS and MS/MS images were obtained from crustacean and rat tissues with pixel size less than 30 mm. Overall, AP/MALDI-Q-Orbitrap is a fast scanning instrument that is capable of performing multiple types of ionization and multiple acquisition modes without instrument modification. This instrument platform provides an attractive alternative to other high resolution MALDI instruments. (C) 2018 Elsevier B.V. All rights reserved.