Renal alterations in prediabletic rats with periodontitis
Renal alterations in prediabletic rats with periodontitis
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DOI：
10.1902/jop.2008.070433
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发表时间：
2008-04-01
影响因子：
4.3
通讯作者：
Flyvbjerg, Allan
中科院分区：
文献类型：
--
作者：
Andersen, Carla C. Pontes;Holmstrup, Palle;Flyvbjerg, Allan
研究背景：牙周炎对糖尿病前期和糖尿病大鼠的血糖水平有影响。Zucker肥胖大鼠（ZFR）是一种典型的糖尿病前期模型，表现为糖耐量受损、高胰岛素血症、血脂异常和中度高血压。本研究的目的是探讨牙周炎是否会影响zfr患者的肾脏变化。方法：对成年雄性ZFRs （N = 19）及其瘦窝仔（N = 18）进行研究。在一半的zfr和瘦鼠中，结扎诱导牙周炎，而另一半作为对照。4周后处死大鼠，取肾、肝、心称重。组织学评估肾脏肾小球体积和肾脏血管内皮生长因子（VEGF）、VEGF受体2、转化生长因子- β、结缔组织生长因子、胶原IV α 1、纤维连接蛋白和肾素mRNA水平。尿白蛋白排泄和肌酐清除率也被评估。结果：在糖尿病前期ZFRs中，牙周炎与肾脏肥大（P= 0.03）和肾小球体积增加的趋势相关（P= 0.06）。在瘦窝鼠中，有牙周炎时，纤维连接蛋白mRNA水平升高（P = 0.03）。结论：我们的研究结果提示牙周炎参与了ZFRs早期肾脏改变的发展。
Background: Periodontitis was shown to have an impact on glucose levels in prediabetic and diabetic rats. The Zucker fatty rat (ZFR) is a well-characterized model of prediabetes presenting with impaired glucose tolerance, hyperinsulinemia, dyslipidemia, and moderate hypertension. The aim of the present study was to investigate whether periodontitis influences kidney changes in ZFRs.Methods: Male adult ZFRs (N = 19) and their lean littermates (N = 18) were studied. Periodontitis was induced with ligatures in half of the ZFRs and lean rats, whereas the other half served as controls. After 4 weeks, the rats were sacrificed, and the kidneys, liver, and heart were removed and weighed. Kidneys were evaluated histologically for glomerular volume and renal mRNA levels of vascular endothelial growth factor (VEGF), VEGF receptor 2, transforming growth factor-beta, connective tissue growth factor, collagen IV alpha 1, fibronectin, and nephrin. Urinary albumin excretion and creatinine clearance were also evaluated.Results: In prediabetic ZFRs, periodontitis was associated with kidney hypertrophy (P = 0.03) and a tendency for increased glomerular volume (P= 0.06). In lean littermates, elevated fibronectin mRNA levels (P = 0.03) were noted in the presence of periodontitis.Conclusion: Our findings suggest the participation of periodontitis in the development of early renal changes in ZFRs.