Quantitation of HIV-1-specific cytotoxic T lymphocytes and plasma load of viral RNA
Quantitation of HIV-1-specific cytotoxic T lymphocytes and plasma load of viral RNA
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DOI：
10.1126/science.279.5359.2103
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发表时间：
1998-03-27
期刊：
影响因子：
56.9
通讯作者：
McMichael, AJ
中科院分区：
文献类型：
--
作者：
Ogg, GS;Jin, X;McMichael, AJ
尽管细胞毒性T淋巴细胞（CTL）被认为参与人类免疫缺陷病毒1型（HIV-1）感染的控制，但尚不可能证明CTL活性与血浆RNA病毒载量之间的直接关系。人白细胞抗原-肽四聚体复合物提供了直接离体定量循环CTL的特定方法。随着四聚体复合物的使用，HIV特异性CTL频率和血浆RNA病毒载量之间观察到显著的负相关。与此相反，没有显着的关联之间检测到生产性感染细胞的清除率和HIV特异性CTL的频率。这些数据与HIV特异性CTL在控制HIV感染中的重要作用一致，并表明病毒在体内具有相当大的致细胞病变作用。
Although cytotoxic T lymphocytes (CTLs) are thought to be involved in the control of human immunodeficiency virus-type 1 (HIV-1) infection, it has not been possible to demonstrate a direct relation between CTL activity and plasma RNA viral load. Human leukocyte antigen-peptide tetrameric complexes offer a specific means to directly quantitate circulating CTLs ex vivo. With the use of the tetrameric complexes, a significant inverse correlation was observed between HIV-specific CTL frequency and plasma RNA viral load. In contrast, no significant association was detected between the clearance rate of productively infected cells and frequency of HIV-specific CTLs. These data are consistent with a significant role for HIV-specific CTLs in the control of HIV infection and suggest a considerable cytopathic effect of the virus in vivo.