Immunoglobulin VH gene structure and diversity in Heterodontus, a phylogenetically primitive shark.
Immunoglobulin VH gene structure and diversity in Heterodontus, a phylogenetically primitive shark.
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异齿鲨（一种系统发育原始鲨鱼）的免疫球蛋白 VH 基因结构和多样性。
DOI：
10.1073/pnas.82.7.2082
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发表时间：
1985
影响因子：
11.1
通讯作者：
Erickson,BW
中科院分区：
文献类型：
--
作者：
Litman,GW;Berger,L;Murphy,K;Litman,R;Hinds,K;Erickson,BW
已使用小鼠VH探针来鉴定和分离Heterodontus francisci（角鲨）DNA文库中的VH同源物。已经确定了一个VH基因HXIA的完整核苷酸序列，并发现其与哺乳动物原型VH基因表现出惊人的组织同源性和核苷酸同一性。完整序列的度量分析是一致的框架和互补决定区（CDR）的早期系统发育多样化。预测的氨基酸序列和特异性杂交的CDR2特异性，合成的寡脱氧核苷酸探针在脾mRNA表明，HXIA的功能表达。由该基因的整个编码区组成的探针与Heterodontus基因组DNA的Southern印迹分析中的多个组分杂交，并且与另外的独特VH +-lambda克隆的鉴定一起表明相当大的复杂性与代表脊椎动物系统发育早期阶段的当代物种中的种系VH基因家族相关。
A mouse VH probe has been used to identify and isolate VH homologs in a DNA library of Heterodontus francisci (horned shark). The complete nucleotide sequence of one VH gene, HXIA, has been determined and found to exhibit striking organizational homology and nucleotide identity with mammalian prototype VH genes. Metric analysis of the complete sequence is consistent with the early phylogenetic diversification of framework and complementarity-determining regions (CDR). Both the predicted amino acid sequence and the specific hybridization of the CDR2-specific, synthetic oligodeoxynucleotide probe in spleen mRNA suggest that HXIA is functionally expressed. A probe consisting of the entire coding region of this gene hybridizes with multiple components in Southern blot analysis of Heterodontus genomic DNA and together with the identification of additional unique VH+-lambda clones indicates that considerable complexity is associated with the germline VH gene family in a contemporary species that represents an early stage in the phylogenetic development of the vertebrates.