Reverse gyrase is not a prerequisite for hyperthermophilic life
Reverse gyrase is not a prerequisite for hyperthermophilic life
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DOI：
10.1128/jb.186.14.4829-4833.2004
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发表时间：
2004-07-01
影响因子：
3.2
通讯作者：
Imanaka, T
中科院分区：
文献类型：
--
作者：
Atomi, H;Matsumi, R;Imanaka, T
We disrupted the reverse gyrase gene from a hyperthermophilic archaeon, Thermococcus kodakaraensis KOD1 An apparent positive supercoiling activity that was observed in the host strain was not found in the disruptant strain. We found that a lack of reverse gyrase led to a retardation in growth that was more striking at higher temperatures. However, the disruption of the reverse gyrase gene did not lead to a lethal phenotype at 90degreesC. This study provides experimental evidence that reverse gyrase is not a prerequisite for hyperthermophilic life.
