Optical coherence tomography angiography for noninvasive evaluation of angiogenesis in a limb ischemia mouse model
Optical coherence tomography angiography for noninvasive evaluation of angiogenesis in a limb ischemia mouse model
复制标题
光学相干断层扫描血管造影用于无创评估肢体缺血小鼠模型的血管生成
DOI：
10.1038/s41598-019-42520-3
复制
发表时间：
2019-04-12
影响因子：
4.6
通讯作者：
Li, Yuejie
中科院分区：
文献类型：
--
作者：
Wang, Liwei;Chen, Zuoguan;Li, Yuejie
We developed an optical coherence tomography angiography technique by improving the speckle contrast algorithm and the imaging process. This technique, which can achieve angiogenesis imagingin vivowithout increasing trauma, was used to evaluate the microvasculature in limb ischemia mice. Sixteen left hindlimb ischemia mice were randomly allocated into CuSO4and saline groups. Within 7 days after treatment, limb ischemic damage, temperature and histological staining were assessed by traditional methods. In addition, angiogenesis was evaluated using an optical coherence tomography angiography systemin vivo. All results were compared. After 7 days of treatment, both the ischemic tissue damage score and temperature ratio of the CuSO4group were significantly higher than those of the control group (all P < 0.05). The number of CD31-positive endothelial cells in the CuSO4group (0.1836 ± 0.0153) was significantly greater than that in the saline control group (0.0436 ± 0.0069) (P < 0.001). Optical coherence tomography angiography showed that the vessel area density of mice in the CuSO4group (0.2566 ± 0.0060) was significantly greater than that of mice in the control group (0.2079 ± 0.0202) (P = 0.027). Optical coherence tomography angiography represents a practical and effective method for observing angiogenesis in the mouse hindlimbin vivowithout increasing trauma.
