Identification of key genes in the response to Salmonella enterica Enteritidis, Salmonella enterica Pullorum, and poly(I:C) in chicken spleen and caecum.
Identification of key genes in the response to Salmonella enterica Enteritidis, Salmonella enterica Pullorum, and poly(I:C) in chicken spleen and caecum.
复制标题
鸡脾和盲肠中对肠炎沙门氏菌、鸡白痢沙门氏菌和 Poly(I:C) 反应的关键基因的鉴定
DOI：
10.1155/2014/154946
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发表时间：
2014
影响因子：
--
通讯作者：
Chen G
中科院分区：
文献类型：
--
作者：
Ma T;Chang G;Chen R;Sheng Z;Dai A;Zhai F;Li J;Xia M;Hua D;Xu L;Wang H;Chen J;Liu L;Chen G
肠炎沙门氏菌(S.Enteritidis)和鸡白肺沙门氏菌(S.Pullorum)被认为是对家禽生产的威胁。这项研究的目的是描述在三种不同挑战下的表达谱特征，并识别鸡脾和盲肠中的感染相关基因。用肠炎沙门氏菌、鸡白肺沙门氏菌或Poly(I：C)对中国鸡种狼山鸡群进行攻击。于感染后7d检测各组小鼠盲肠和肝脏细胞因子和抗体浓度及沙门氏菌定植水平。利用从脾和盲肠中分离的mRNA进行表达芯片实验。鉴定了与免疫相关的关键差异表达基因(Deg)。在三个攻击组(RBM16、FAH、SOX5和RBM9)的脾中发现了四个DEG，在盲肠中发现了四个不同的基因(SOURE、FCN2、ANLN和ACSL1)。三组受试者的表情特征明显不同。感染鸡白破沙门氏菌后，脾中富集基因主要集中在淋巴细胞增殖相关途径中，而盲肠中富集基因主要集中在先天免疫或抗菌反应中。在所有三个挑战组中出现的DEG可能代表了不同病原体的全球反应因素。
Salmonella enterica Enteritidis (S. Enteritidis) and Salmonella enterica Pullorum (S. pullorum) are regarded as a threat to poultry production. This study's aim is to characterize the expression profiles in response to three different challenges and to identify infection-related genes in the chicken spleen and caecum. Groups of the Chinese chicken breed Langshan were challenged with either S. Enteritidis, S. pullorum, or poly(I:C). The concentrations of cytokines and antibodies and the Salmonella colonization level of the caecum and liver were detected in each group at 7 days postinfection. Expression microarray experiments were conducted using mRNA isolated from both spleen and caecum. Crucial differentially expressed genes (DEGs) associated with immunity were identified. Four DEGs were identified in spleen of all three challenge groups (RBM16, FAH, SOX5, and RBM9) and different four genes in caecum (SOUL, FCN2, ANLN, and ACSL1). Expression profiles were clearly different among the three challenged groups. Genes enriched in the spleen of birds infected with S. pullorum were enriched in lymphocyte proliferation related pathways, but the enriched genes in the caecum of the same group were primarily enriched in innate immunity or antibacterial responses. The DEGs that appear across all three challenge groups might represent global response factors for different pathogens.
影响因子：
2.1
作者：
Shimizu N;Noda S;Katayama K;Ichikawa H;Kodama H;Miyoshi H
通讯作者：
Miyoshi H