Autoimmune epitopes in messenger RNA.
Autoimmune epitopes in messenger RNA.
复制标题
信使 RNA 中的自身免疫表位。
DOI：
10.1017/s1355838202021507
复制
发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Keene,JackD
中科院分区：
文献类型：
--
作者：
Lipes,BarbaraD;Keene,JackD
Patients with systemic autoimmune disorders produce autoantibodies against sequence-specific conformational RNA epitopes on U1 snRNA, 28S rRNA, and transfer RNAs. The molecular basis for immunological reactivity with these highly abundant and stable RNAs is not understood. Here, we report the existence of discrete RNA epitopes in messenger RNAs that are generally less abundant and less stable than snRNAs and tRNAs. An iterative selection and amplification procedure using pooled autoimmune patient sera identified immunoreactive mRNA species. Following deconvolution of the pools to identify the reactive sera, several mRNAs recognized by these autoantibodies were cloned and sequenced. Detailed analysis using one particular serum indicated reactivity against the messages encoding alternative splicing factor (ASF/SF2) and calmodulin. Deletion and site-directed mutagenesis determined that an epitope recognized by this serum is located in a 17-base stem-loop structure common to both messages. This serum was then used to immunoprecipitate native mRNAs encoding ASF/SF2 and calmodulin from total HeLa cell RNA. Our results demonstrate that despite its low abundance and instability, messenger RNA is capable of reacting with autoantibodies generated during an autoimmune response. These data are consistent with direct presentation as a model to explain the generation of RNA conformation-specific autoantibodies.
登录
查看更多内容
自身抗原和自身抗体对 RNA 的识别
影响因子：
2.8
作者：
J. Keene
通讯作者：
J. Keene
RNA 表面作为蛋白质的功能模拟物。
影响因子：
--
作者：
J. Keene
通讯作者：
J. Keene
结缔组织病患者抗小核核糖核蛋白血清中的抗 (U1) 小核 RNA 抗体。
影响因子：
15.9
作者：
W. V. van Venrooij;R. Hoet;J. Castrop;B. Hageman;I W Mattaj;L. B. A. van de Putte
通讯作者：
L. B. A. van de Putte
影响因子：
14.9
作者：
Hamm, J
通讯作者：
Hamm, J
核自身抗原的分子生物学。
DOI：
--
发表时间：
1993
期刊：
Advances in nephrology from the Necker Hospital
影响因子：
--
作者：
Tan,EM
通讯作者：
Tan,EM