ISOLATION OF A CANDIDATE HUMAN HEMATOPOIETIC STEM-CELL POPULATION
ISOLATION OF A CANDIDATE HUMAN HEMATOPOIETIC STEM-CELL POPULATION
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DOI：
10.1073/pnas.89.7.2804
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发表时间：
1992-04-01
影响因子：
11.1
通讯作者：
PEAULT, B
中科院分区：
文献类型：
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作者：
BAUM, CM;WEISSMAN, IL;PEAULT, B
我们已经鉴定出一种罕见的(0.05-0.1%)人胎儿骨髓细胞亚群，它含有多能造血祖细胞。表达Thy-1和CD34但没有已知谱系标志的人类前体细胞群体具有丰富的克隆形成活性，从而在小鼠骨髓基质系上建立长期的多谱系(粒单核细胞和B淋巴系)培养。此外，Thy-1+CD34+亚群几乎不含荧光线粒体染料罗丹明123，它包含了几乎所有建立长期培养的细胞。将人胎胸腺移植到严重联合免疫缺陷(SCID)小鼠体内，Thy-1+CD34+胎鼠骨髓细胞分化为T淋巴细胞。在9例中的2例中，同种异体Thy-1+CD34+细胞可以移植到在SCID小鼠中生长的完整的人胎儿骨髓中，从而产生供者来源的髓系和B细胞。通过外推，罕见的人类Thy-1+Lin-CD34+细胞群含有多能造血祖细胞；我们认为它对于候选造血干细胞是高度浓缩的。
We have identified a rare (0.05-0.1%) subset of human fetal bone marrow cells that contains multipotent hematopoietic precursors. The population of human precursor cells that express Thy-1 and CD34 but no known lineage markers is enriched for clonogenic activity that establishes long-term, multilineage (myelomonocytic and B lymphoid) cultures on mouse marrow stromal lines. Further, the Thy-1+CD34+ subset that takes up little of the fluorescent mitochondrial dye rhodamine 123 contains virtually all the cells that establish long-term cultures. In human fetal thymus transplanted into SCID (severe combined immunodeficiency) mice, Thy-1+CD34+ fetal bone marrow cells differentiate into T lymphocytes. In two of nine cases, allogeneic Thy-1+CD34+ cells could engraft intact human fetal bone marrow grown in SCID mice, resulting in donor-derived myeloid and B cells. By extrapolation, the rare human Thy-1+Lin-CD34+ cell population contains pluripotent hematopoietic progenitors; we propose that it is highly enriched for candidate hematopoietic stem cells.