Inhibition of the binding of progesterone receptor to nuclei: effects of o-phenanthroline and rifamycin AF/013.
Inhibition of the binding of progesterone receptor to nuclei: effects of o-phenanthroline and rifamycin AF/013.
复制标题
黄体酮受体与细胞核结合的抑制：邻菲咯啉和利福霉素 AF/013 的作用。
DOI：
10.1016/0006-291x(75)90275-2
复制
发表时间：
1975
影响因子：
3.1
通讯作者：
David O. Toft
中科院分区：
文献类型：
--
作者：
Phoebe Lohmar;David O. Toft
当用[3 H]孕酮标记的鸡输卵管孕酮受体制剂与邻菲咯啉或利福霉素AF/013适当孵育时，[3 H]孕酮受体与纯化的输卵管核结合的能力几乎完全消失，尽管类固醇受体复合物本身基本上保持完整。间菲咯啉和利福平不引起核结合能力的显著抑制。这些研究结果进行了讨论，在有关已知的影响相同的化合物对核酸聚合酶。邻菲咯啉的有效性表明受体可能是金属蛋白，其中金属离子参与受体与核结合位点的连接。
When preparations of chick oviduct progesterone receptor, labeled with [3H]progesterone, are suitably incubated with o-phenanthroline or rifamycin AF/013, the ability of the [3H]progesterone-receptor to bind to purified oviduct nuclei is almost completely abolished, although the steroid-receptor complex itself remains essentially intact. m-Phenanthroline and rifampicin do not cause significant inhibition of nuclear-binding ability. These findings are discussed in relation to known effects of the same compounds on nucleic acid polymerases. The effectiveness of o-phenanthroline suggests that the receptor may be a metalloprotein in which the metal ion participates in the attachment of receptor to nuclear binding sites.