Seminal proteins but not sperm induce morphological changes in the Drosophila melanogaster female reproductive tract during sperm storage
Seminal proteins but not sperm induce morphological changes in the Drosophila melanogaster female reproductive tract during sperm storage
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DOI：
10.1016/j.jinsphys.2006.12.003
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发表时间：
2007-04-01
影响因子：
2.2
通讯作者：
Wolfner, Mariana F.
中科院分区：
文献类型：
--
作者：
Adams, Erika M.;Wolfner, Mariana F.
在大多数昆虫中，雄性在交配过程中转移给雌性的精子储存在雌性生殖道内，以供随后受精时使用。在果蝇中，精液中的雄性辅助腺蛋白 (Acps) 是精子在雌性精子储存器官中有效积累所必需的。为了确定精子储存过程中雌性生殖道内发生的事件，以及 Acps 和精子在这些事件中所起的作用，我们确定了与野生型、Acp 缺陷或精子缺陷雄性交配的雌性在精子储存过程中发生的形态变化。野生型交配中会发生一系列可重复的形态变化。这些被分为 10 个刻板阶段。女性在这些阶段的进展中不需要精子，但接受 Acps 对于形态变化的前几个阶段的进展至关重要。此外，接受少量 Acps 的雌性比未接受 Acps 的雌性到达的阶段稍晚。我们的结果表明，成功储存精子可能需要雌性生殖道（可能本质上是肌肉性的）及时的形态变化，并且需要来自雄性的Acps才能发生这些变化。 (c) 2007 Elsevier Ltd. 保留所有权利。
in most insects, sperm transferred by the male to the female during mating are stored within the female reproductive tract for subsequent use in fertilization. In Drosophila melanogaster, male accessory gland proteins (Acps) within the seminal fluid are required for efficient accumulation of sperm in the female's sperm storage organs. To determine the events within the female reproductive tract that occur during sperm storage, and the role that Acps and sperm play in these events, we identified morphological changes that take place during sperm storage in females mated to wild-type, Acp-deficient or sperm-deficient males. A reproducible set of morphological changes occurs in a wild-type mating. These were categorized into 10 stereotypic stages. Sperm are not needed for progression through these stages in females, but receipt of Acps is essential for progression beyond the first few stages of morphological change. Furthermore, females that received small quantities of Acps reached slightly later stages than females that received no Acps. Our results suggest that timely morphological changes in the female reproductive tract, possibly Muscular in nature, may be needed for successful sperm storage, and that Acps from the male are needed in order for these changes to occur. (c) 2007 Elsevier Ltd. All rights reserved.