Identification and expression of odorant-binding proteins of the malaria-carrying mosquitoes Anopheles gambiae and Anopheles arabiensis
Identification and expression of odorant-binding proteins of the malaria-carrying mosquitoes Anopheles gambiae and Anopheles arabiensis
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DOI：
10.1002/arch.20047
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发表时间：
2005-03-01
影响因子：
2.2
通讯作者：
Zhou, JJ
中科院分区：
文献类型：
--
作者：
Li, ZX;Pickett, JA;Zhou, JJ
寄主偏好和取血仅限于雌性蚊子。嗅觉在寻找宿主的行为中起着重要作用，这可能与蚊子嗅觉基因的一个子集有关。参与嗅觉的蛋白质包括气味受体(ORs)和气味结合蛋白(OBP)。OBP被认为是昆虫触角内的载体，将发生的物质输送到嗅觉感受器。在这里，我们报道了32个编码冈比亚按蚊OBP的基因的注释，以及它们在两种高度亲人的按蚊复合体蚊种中的组织特异性表达。配子严格意义上是一种机会主义的，但更嗜食动物的物种，一种。阿罗比安。RT-PCR显示，其中一些基因主要在头部组织中表达，其中一部分在女性头部中表达最高。其中一个基因(AgCP1588)与果蝇信息素结合蛋白4(PBPRP4)有很高的相似性(40%)，且仅在两个AN的头部表达，尚未被鉴定为OBP。Gombiae和An.阿拉伯，并在较高水平的女性头部。两个基因(agCP3071和agCP15554)仅在雌性头部表达，agC15554也在雌性头部有较高的表达水平。冈比亚亚目。按蚊复合体两个成员的基因表达谱为进一步分析蚊子嗅觉器官提供了第一步。拱门。昆虫生物(哼哼。物理。2005年，58：175-189。(C)2005年Wiley-Liss，Inc.
Host preference and blood feeding are restricted to female mosquitoes. Olfaction plays a major role in host-seeking behaviour, which is likely to be associated with a subset of mosquito olfactory genes. Proteins involved in olfaction include the odorant receptors (ORs) and the odorant-binding proteins (OBPs). OBPs are thought to function as a carrier within insect antennae for transporting occurs to the olfactory receptors. Here we report the annotation of 32 genes encoding putative OBPs in the malaria mosquito Anopheles gambiae and their tissue-specific expression in two mosquito species of the Anopheles complex, a highly anthropophilic species An. gambioe sensu stricto and an opportunistic, but more zoophilic species, An. arobiensis. RTPCR shows that some of the genes are expressed mainly in head tissue and a subset of these show highest expression in female heads. One of the genes (agCP1588) which has not been identified as an OBP, has a high similarity (40%) to the Drosophila pheromone- binding protein 4 (PBPRP4) and is only expressed in heads of both An. gombiae and An. arabiensis, and at higher levels in female heads. Two genes (agCP3071 and agCP15554) are expressed only in female heads and agC15554 also shows higher expression levels in An. gambiae. The expression profiles of the genes in the two members of the Anopheles complex provides the first step towards further molecular analysis of the mosquito olfactory apparatus. Arch. Insect Bio(hem. Physiol. 58:175-189, 2005. (C) 2005 Wiley-Liss, Inc.