Potential biomarkers in the urine of myocardial infarction rats: a metabolomic method and its application
Potential biomarkers in the urine of myocardial infarction rats: a metabolomic method and its application
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DOI：
10.1039/c0mb00110d
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发表时间：
2011-01-01
影响因子：
--
通讯作者：
Zhang, Weidong
中科院分区：
文献类型：
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作者：
Jiang, Peng;Dai, Weixing;Zhang, Weidong
开发了一种使用反相液相色谱/四极杆质谱（LC-Q-TOF-MS）的代谢组方法，以获得心肌梗死（MI）的发展和进展的系统视图。通过与部分最小二乘判别分析（PLS-DA）结合，确定了大鼠尿液中的16个生物标志物，其中8个与能量代谢途径有关。在受调节的途径中，柠檬酸周期相关的网络受到敏锐的干扰。代谢组结果不仅提供了MI发展和发展的系统观点，而且还为预防或治疗MI提供了理论基础。该开发的方法还用于分析中药（TCM）的治疗作用，名为Shexiang Baoxin Pill（SBP），这是一种广泛使用的抗MI药物。结果表明，SBP给药可以通过部分调节能量代谢的扰动途径来对MI产生令人满意的影响。
A metabolomic method using reversed-phase liquid chromatography/quadrupole time-of-flight mass spectrometry (LC-Q-TOF-MS) was developed to obtain a systematic view of the development and progression of myocardial infarction (MI). By combining with partial least squares discriminant analysis (PLS-DA), 16 biomarkers in rat urine were identified and eight of them were related to the pathway of energy metabolism. Among the regulated pathways, the citric acid cycle related network was acutely perturbed. The metabolomic results not only supplied a systematic view of the development and progression of MI but also provided the theoretical basis for the prevention or treatment of MI. The developed method was also used to analyze the therapeutic effects of a traditional Chinese medicine (TCM) named Shexiang Baoxin Pill (SBP), a widely used anti-MI medicine in clinics. The results showed that SBP administration could provide satisfactory effects on MI through partially regulating the perturbed pathway of energy metabolism.