Comparison of phenotypes and transcriptomes of mouse skin-derived precursors and dermal mesenchymal stem cells
Comparison of phenotypes and transcriptomes of mouse skin-derived precursors and dermal mesenchymal stem cells
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小鼠皮肤来源的前体细胞和真皮间充质干细胞的表型和转录组比较
DOI：
10.1016/j.diff.2018.07.001
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发表时间：
2018-07-01
期刊：
影响因子：
2.9
通讯作者：
Li, Li
中科院分区：
文献类型：
--
作者：
Li, Yiming;Li, Xiaohua;Li, Li
皮肤源性前体细胞（SKPs）和真皮间充质干细胞（dMSCs）都是细胞治疗和再生医学的有前途的候选者。迄今为止，小鼠SKPs （mSKPs）和dMSCs的表型和转录组的比较尚未报道。在这里，我们对来自同一小鼠真皮样本的mSKP和dMSCs的生物学特性和转录组进行了表征和比较。首先，我们利用免疫细胞化学、细胞周期分析和CD抗原表达分析了mSKPs和dMSCs。然后我们对两种细胞类型进行成骨、脂肪和软骨诱导分化。最后，我们通过rna测序（RNA-Seq）比较了它们的基因组图谱，并通过定量实时反转录PCR （qRT-PCR）验证了RNA-Seq的结果。结果表明，mSKPs和dMSCs在某些阳性干细胞标记物表达上具有相似性，但在Nanog和Oct4表达上存在差异。mSKPs和dMSCs表现出相似的细胞周期分布和CD抗原表达。两种细胞均可诱导分化为骨细胞、脂肪细胞和软骨细胞。然而，RNA-Seq和qRT-PCR结果表明，mSKPs和dMSCs具有不同的转录组谱。从mSKPs富集的大多数差异表达基因（deg）是免疫相关的，而从dMSCs富集的大多数deg是分化/发育/疾病相关的。转录组分析表明，mSKPs和dMSCs可能在相关的发病率管理中具有潜在的用途。这些结果可能为基于干细胞的新型治疗策略提供了分子基础。
Both skin-derived precursors (SKPs) and dermal mesenchymal stem cells (dMSCs) are promising candidates for cellular therapy and regenerative medicine. To date the comparison of phenotypes and transcriptomes of mouse SKPs (mSKPs) and dMSCs has never been reported. Here we characterized and compared the biological properties and transcriptomes of mSKP and dMSCs from the same mouse dermis sample. Firstly, we analyzed mSKPs and dMSCs by use of immunocytochemistry, cell cycle analysis, and CD antigen expression. Then we conducted the osteogenic, adipogenic, and chondrogenic induced differentiation for both cell types. Lastly, we compared their genomic profiles by RNA-sequencing (RNA-Seq), and verified the results of RNA-Seq by quantitative real time reverse transcription PCR (qRT-PCR). The results suggested that mSKPs and dMSCs shared similarities in certain positive stem cells markers expression, but demonstrated difference in Nanog and Oct4 expression. mSKPs and dMSCs demonstrated similar cell cycle distribution and CD antigen expression. Both types of cells could be induced differentiated into osteocytes, adipocytes, and chondrocytes. However, RNA-Seq and qRT-PCR results indicated that mSKPs and dMSCs had distinct transcriptome profiles. The majority of enriched differentially expressed genes (DEGs) from mSKPs was immune-related, while the majority of enriched DEGs from dMSCs was differentiation/development/disease-related. Transcriptome profiles suggested that mSKPs and dMSCs might have potential usage in the relevant morbidity management. These results may indicate a molecular basis for novel stem cell-based therapeutic strategies.