Differential expression of histone deacetylases during banana ripening and identification of MaHDA6 in regulating ripening-associated genes
Differential expression of histone deacetylases during banana ripening and identification of MaHDA6 in regulating ripening-associated genes
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香蕉成熟过程中组蛋白脱乙酰酶的差异表达及MaHDA6调控成熟相关基因的鉴定
DOI：
10.1016/j.postharvbio.2018.03.010
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发表时间：
2018-07-01
影响因子：
7
通讯作者：
Lu, Wang-Jin
中科院分区：
文献类型：
--
作者：
Fu, Chang-Chun;Han, Yan-Chao;Lu, Wang-Jin
组蛋白脱乙酰基酶（HDAC）介导的核心组蛋白的脱乙酰化是植物果实成熟和衰老等多个发育过程中调控基因阻遏的重要机制。香蕉果实的成熟是一个高度协调的发育程序化事件，涉及成熟相关基因的遗传和表观遗传调控。尽管HDAC在模式植物中的生物学功能已被广泛研究，但对香蕉HDAC基因家族的了解非常有限。在香蕉基因组中共鉴定出17个HDAC，根据序列相似性和同源性将其中的12个、2个和3个分别归为RPD 3/HDA 1、SIR 2和HD2亚家族。表达谱显示，这些MaHDAC在香蕉果实成熟过程中差异表达。在这些MaHDAC中，MaHDA 6在果实成熟中显示出最高的诱导，因此被选择用于进一步研究。MaHDA 6定位于细胞核内，在植物细胞中具有组蛋白脱乙酰酶活性。染色质免疫沉淀（ChIP）分析表明，MaHDA 6能够与乙烯信号通路基因MaERF 11/15的启动子结合。特别是MaERF 11/15调控区的组蛋白H3和H4乙酰化水平在成熟期明显降低，这与其表达水平的降低相一致。总之，我们的研究结果表明，不同的MaHDACs在香蕉成熟过程中有不同的表达模式，MaHDA 6可能参与成熟推测通过调节组蛋白脱乙酰化MaERF 11/15的转录。
Deacetylation of core histone proteins mediated by histone decetylases (HDACs) is an important machinery in regulating gene repression in multiple plant developmental processes including fruit ripening and senescence. The ripening of banana fruit is a highly coordinated and developmentally programmed event which involves the genetic and epigenetic regulation of ripening-associated genes. Despite the biological functions of HDACs in model plants have been extensively studied, knowledge about HDAC gene family in banana is very limited. In this work, a total of 17 HDACs were identified in the genome of banana, among which 12, 2 and 3 members are grouped into RPD3/HDA1, SIR2 and HD2 sub-families respectively, based on the sequence similarity and phylogeny. Expression profiling revealed that these MaHDACs were differentially expressed during banana fruit ripening. Of these MaHDACs, MaHDA6 showed the highest inducement in fruit ripening and thus was selected for further study. MaHDA6 was localized within the nucleus and contained histone deacetylase activity in plant cells. Furthermore, chromatin immunoprecipitation (ChIP) assays indicated that MaHDA6 was capable of binding to the promoters of MaERF11/15, the genes in ethylene signaling pathway. Particularly, the levels of acetylation of histones H3 and H4 in MaERF11/15's regulatory regions were obviously decreased at ripening stage, which was in accordance with their decreased expression levels. Taken together, our findings suggest that various MaHDACs have distinct expression patterns during banana ripening and that MaHDA6 might be involved in the ripening presumably via regulating the transcription of MaERF11/15 by histone deacetylation.