NORADRENALINE-INDUCED CALORIGENESIS IN WARM-ACCLIMATED AND IN COLD-ACCLIMATED RATS - RELATIONS BETWEEN CONCENTRATION OF NORADRENALINE IN ARTERIAL PLASMA, BLOOD-FLOW TO DIFFERENTLY LOCATED MASSES OF BROWN ADIPOSE-TISSUE, AND CALORIGENIC RESPONS
NORADRENALINE-INDUCED CALORIGENESIS IN WARM-ACCLIMATED AND IN COLD-ACCLIMATED RATS - RELATIONS BETWEEN CONCENTRATION OF NORADRENALINE IN ARTERIAL PLASMA, BLOOD-FLOW TO DIFFERENTLY LOCATED MASSES OF BROWN ADIPOSE-TISSUE, AND CALORIGENIC RESPONS
复制标题
DOI：
10.1139/y80-140
复制
发表时间：
1980-01-01
影响因子：
2.1
通讯作者：
FRYDMAN, ML
中科院分区：
文献类型：
--
作者：
FOSTER, DO;DEPOCAS, F;FRYDMAN, ML
巴比妥镇静，热适应（WA）或冷适应（CA）大鼠静脉注射去甲肾上腺素（NA，去甲肾上腺素）的剂量，引起分级产热反应。血流量（Q）的各种机构的棕色脂肪组织（BAT），NA诱导的产热的主要网站，测量与标记的微球。测定肩胛间BAT动脉血、静脉血O2含量及动脉血浆NA浓度。ANA与NA剂量呈线性相关。产热反应、BAT总Q值和BAT单体Q值均为ANA的S型函数。产热反应或增加流向BAT的流量的阈值是ANA = 0.0000。2 ng/mL（12 nM），CA大鼠中BAT的一些小体除外，其接近4 ng/mL。总BAT和产热反应的O2输送呈线性相关。BAT小体在Q/g上呈异质性，CA大鼠的Q/g层次随ANA和产热反应的增加而明显改变。BAT中的产热通常不由循环NA介导，在NA输注的大鼠中，神经元和神经元对NA的摄取将使BAT的肾上腺素受体处的NA浓度低于循环中的NA浓度，并且许多因素，即，肾上腺素能神经支配的组织和密度以及受体的数量和功效一定有助于确定BAT的测量反应。大鼠体内BAT的不同部位在组成和结构上可能存在显著差异，在冷适应过程中可能发生不同的发育。
Barbital-sedated, warm-acclimated (WA) or cold-acclimated (CA) rats were infused i.v. with noradrenaline (NA, norepinephrine) at doses that elicited graded calorigenic responses. Blood flow (Q) to the various bodies of brown adipose tissue (BAT), the major sites of the NA-induced calorigenesis, was measured with labeled microspheres. The O2 content of arterial blood and of venous blood from interscapular BAT and the concentration of NA in arterial plasma (ANA) were determined. ANA was linearly related to the dose of NA. Calorigenic response and the Q of total BAT and of separate bodies of BAT were sigmoid functions of ANA. The threshold for calorigenic response or for increased flow to BAT was an ANA of .apprx. 2 ng/mL (12 nM), except for some bodies of BAT in CA rats where it was closer to 4 ng/ml. Delivery of O2 to total BAT and calorigenic response were related linearly. The bodies of BAT were heterogenous in Q/g and in CA rats the hierarchy in Q/g changed markedly as ANA and calorigenic response increased. Calorigenesis in BAT normally is not mediated by circulating NA, that in NA-infused rats neuronal and extraneuronal uptakes of NA would effect a lower concentration of NA at the adrenoceptors of BAT than in the circulation and that many factors, i.e., organization and density of adrenergic innervation and the number and efficacy of receptors must have contributed to determining the measured responses of BAT. The differently located bodies of BAT in rats may have significant differences in composition and structure and that they may undergo differential development during cold acclimation.