Transcriptome analysis of an invasive weed Mikania micrantha
Transcriptome analysis of an invasive weed Mikania micrantha
复制标题
入侵杂草薇甘菊的转录组分析
DOI：
10.1007/s10535-012-0024-3
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发表时间：
2012-03-01
影响因子：
1.5
通讯作者：
Shi, S. -H.
中科院分区：
文献类型：
--
作者：
Huang, Y. -L.;Fang, X. -T.;Shi, S. -H.
作为了解植物成为入侵的分子机制的第一步，我们在这里提出了第一个转录组分析的入侵杂草薇甘菊。该分析基于由Illumina Genome Analyzer II系统生成的75个核苷酸短读段数据。基于单个M的转录组的870万个过滤的配对末端序列读数，从头组装了总共31131个独特序列。在田野里生长的薇甘菊。73%的独特序列与NCBI数据库中已有的蛋白质具有显著的相似性，21 448个序列可以基于基因本体分配进行分组。特别感兴趣的是与基因组进化、可塑性、次级代谢和防御反应中涉及的基因共享同源性的序列。
As an initial step towards understanding the molecular mechanisms by which plants become invasive, we present here the first transcriptome analysis for an invasive weed Mikania micrantha. The analysis was based on the 75-nucleotide short reads data generated by the Illumina Genome Analyzer II system. A total of 31 131 unique sequences were assembled de novo based on 8.7 million filtered paired-end sequence reads for the transcriptome of an individual M. micrantha growing in the field. 73 % of the unique sequences showed significant similarity to existing proteins in the NCBI database, and 21 448 could be grouped based on gene ontology assignments. Of particular interest are the sequences that share homology with genes involved in genome evolution, plasticity, secondary metabolism and defense responses.