Temporal regulation of microRNA expression in Drosophila melanogaster mediated by hormonal signals and Broad-Complex gene activity
Temporal regulation of microRNA expression in Drosophila melanogaster mediated by hormonal signals and Broad-Complex gene activity
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DOI：
10.1016/s0012-1606(03)00208-2
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发表时间：
2003-07-01
影响因子：
2.7
通讯作者：
Ambros, V
中科院分区：
文献类型：
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作者：
Sernpere, LF;Sokol, NS;Ambros, V
LIN-4和LET-7是一个广泛的基因家族的创始成员，这些基因产生小的转录本，被称为microRNAs(MiRNAs)。在秀丽线虫中，LIN-4和LET-7通过翻译抑制靶基因来控制胚胎后事件的发生时间，允许从早期发育程序到晚期发育程序的进展。为了寻找在果蝇发育调控中发挥类似作用的miRNAs，我们研究了24个miRNAs在果蝇体内的发育表达谱，发现了7个在变态过程中表达上调或下调的miRNAs。其中三个miRNAs(mir-100、mir-125和let-7)的上调和第四个miRNAs(mir-34)的下调需要激素蜕皮激素(ECD)和ECD诱导基因Bide-Complex的活性。有趣的是，mir-125可能是LIN-4的同源物。MiR-100、-125和let-7聚集在2L染色体上一个800bp的区域内，这表明这三个miRNAs在变态过程中可能通过共同的顺式作用元件受到协同调控。在S2细胞中，ECD和保幼激素类似物美托品对这四种miRNAs的表达有相反的影响，表明这两种激素都参与了体内mir-34、-100、-125和let-7表达的时间调控。(C)2003年埃尔塞维尔科学公司(美国)。版权所有。
lin-4 and let-7 are founding members of an extensive family of genes that produce small transcripts, termed microRNAs (miRNAs). In Caenorhabditis elegans, lin-4 and let-7 control the timing of postembryonic events by translational repression of target genes, permitting progression from early to late developmental programs. To identify Drosophila melanogaster miRNAs that could play similar roles in the control of developmental timing, we characterized the developmental expression profile of 24 miRNAs in Drosophila, and found 7 miRNAs that are either upregulated or downregulated in conjunction with metamorphosis. The upregulation of three of these miRNAs (mir-100, mir-125, and let-7), and the downregulation of a fourth (mir-34) requires the hormone ecdysone (Ecd) and the activity of the Ecd-inducible gene Broad-Complex. Interestingly, mir-125 is a putative homologue of lin-4. mir-100, -125, and let-7 are clustered within an 800-bp region on chromosome 2L, suggesting that these three miRNAs may be coordinately regulated via common cis-acting elements during metamorphosis. In S2 cells, Ecd and the juvenile hormone analog methoprene exert opposite effects on the expression of these four miRNAs, indicating the participation of both these hormones in the temporal regulation of mir-34, -100, -125, and let-7 expression in vivo. (C) 2003 Elsevier Science (USA). All rights reserved.