Epithelial induction of porcine suppressor of cytokine signaling 2 (SOCS2) gene expression in response to Entamoeba histolytica
Epithelial induction of porcine suppressor of cytokine signaling 2 (SOCS2) gene expression in response to Entamoeba histolytica
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DOI：
10.1016/j.dci.2009.12.017
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发表时间：
2010-05-01
影响因子：
2.9
通讯作者：
Meurens, Francois
中科院分区：
文献类型：
--
作者：
Bruel, Timothee;Guibon, Roseline;Meurens, Francois
细胞因子信号抑制蛋白（SOCS）是先天免疫和适应性免疫的关键生理调节因子。这些蛋白属于细胞因子信号的三大类调节剂。在接下来的文章中，我们利用猪极化肠细胞研究了对原生动物溶组织内阿米巴的早期反应，并通过快速扩增cDNA末端（RACE） PCR鉴定了猪SOCS1、SOCS4、SOCS5和SOCS6的编码序列。预测猪SOCS蛋白与人类SOCS蛋白的相似性超过92%。在SOCS转录本中，只有SOCS2 mRNA在肠上皮细胞中被显著诱导，以响应寄生虫的细胞溶解活性。极化肠细胞与溶组织芽胞杆菌共培养3小时后获得的转录组学特征明显倾向于炎症和中性粒细胞的募集。这些转录组数据通过使用多个经过验证的内参基因进行了规范化。该分析为今后猪肠细胞的研究提供了第一组有用的参考基因。我们的数据揭示了极化肠细胞对溶组织芽胞杆菌的早期反应，并确定了SOCS2可能参与寄生虫对宿主反应的调节。(C) 2010 Elsevier Ltd.版权所有。
Suppressor of cytokine signaling (SOCS) proteins are key physiological regulators of both innate and adaptive immunity. These proteins belong to the three major classes of modulators of cytokines signaling. In the following article, we used porcine polarized intestinal cells to study early response to the protozoan, Entamoeba histolytica, and we identified by rapid amplification of cDNA ends (RACE) PCR porcine SOCS1, SOCS4, SOCS5 and SOCS6 encoding sequences. With more than 92% identity predicted porcine SOCS proteins are very similar to their human counterparts. Among SOCS transcripts, only SOCS2 mRNA was significantly induced in epithelial intestinal cells in response to the cytolytic activity of the parasite. The transcriptomic profile obtained after 3 h of co-culture of polarized intestinal cells with E. histolytica was clearly oriented toward inflammation and the recruitment of neutrophils. These transcriptomic data have been normalized with accuracy by the utilisation of multiple validated reference genes. The analysis offers a first set of reference genes useful for future studies in porcine intestinal cells. Our data shed light on the understanding of the early response of polarized intestinal cells to E. histolytica and identified a potential involvement of SOCS2 in the parasite regulation of the host response. (C) 2010 Elsevier Ltd. All rights reserved.