Outer membrane-anchoring enables LpoB to regulate peptidoglycan synthesis rate.
Outer membrane-anchoring enables LpoB to regulate peptidoglycan synthesis rate.
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DOI：
10.1016/j.tcsw.2022.100086
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发表时间：
2022-12
期刊：
影响因子：
--
通讯作者：
Vollmer, Waldemar
中科院分区：
文献类型：
--
作者：
Kermani, Ali A.;Biboy, Jacob;Vollmer, Daniela;Vollmer, Waldemar
关键词：
错误定位的LpoB能够激活PBP 1B。LpoB错误定位影响渗透上移后的细胞生长。LpoB错误定位改变细胞形态后，渗透上移。肽聚糖组成不受LpoB错误定位的显著影响。LpoB的错误定位影响PG合成速率后，渗透上移。肽聚糖（PG）是大多数细菌中细胞包膜的重要组成部分，负责维持细胞的形状并保护细胞免受环境胁迫。PG层的生长在细胞伸长和分裂过程中是由PG脱氢酶和水解酶的协调活动促进的。PG脱氢酶由细胞内的延长酶体和分裂体复合物的组分调节，这些复合物由细胞骨架蛋白MreB和FtsZ驱动。在大肠杆菌中，PG脱氢酶PBP 1A和PBP 1B需要分别被外膜（OM）锚定的脂蛋白LpoA和LpoB激活。这些具有伸长的结构，并且能够跨越周质以通过PG层到达其同源的细胞质膜（CM）锚定的PG合酶。据推测，Lpo蛋白在PG网被拉伸或有缺陷的位点激活PBP，导致PG合酶激活与细胞生长或PG修复偶联。在这里，我们调查的重要性OM锚定的功能的LPO蛋白在调节PG合成响应环境压力。我们研究了人工CM-栓系的LpoB对细胞形态和PG合成的影响。我们的研究结果表明，LpoB的错误定位影响细胞在高渗透压生长培养基中的生长和形态，以及渗透压升高后PG合成速率。
Mis-localized LpoB is able to activate PBP1B. LpoB mis-localization affects cell growth upon osmotic upshift. LpoB mis-localization alters the cell morphology upon osmotic upshift. Peptidoglycan composition is not significantly affected by mis-localization of LpoB. Mis-localization of LpoB affects PG synthesis rate after an osmotic upshift. Peptidoglycan (PG) is an essential component of the cell envelope in most bacteria, responsible for maintaining the shape of the cell and protecting the cell from environmental stresses. The growth of the PG layer during cell elongation and division is facilitated by the coordinated activities of PG synthases and hydrolases. PG synthases are regulated from inside the cell by components of the elongasome and divisome complexes driven by the cytoskeletal proteins MreB and FtsZ. In Escherichia coli the PG synthases PBP1A and PBP1B require the activation by outer membrane (OM)-anchored lipoproteins LpoA and LpoB, respectively. These have an elongated structure and are capable to span the periplasm to reach their cognate, cytoplasmic membrane (CM)-anchored PG synthase through the PG layer. Presumably, the Lpo proteins activate the PBPs at sites where the PG mesh is stretched or defective, resulting in coupling of PG synthase activation with cell growth or PG repair. Here we investigated the importance of OM-anchoring on the function of Lpo proteins in regulating PG synthesis in response to environmental stresses. We investigated the effects of an artificially CM-tethered LpoB on cell morphology and PG synthesis. Our results indicate that mis-localization of LpoB affects the growth and morphology of cells in high osmolarity growth medium, and PG synthesis rate upon an osmotic upshift.
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影响因子：
15
作者：
Lupoli TJ;Lebar MD;Markovski M;Bernhardt T;Kahne D;Walker S
通讯作者：
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影响因子：
3.4
作者：
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DOI：
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发表时间：
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期刊：
Cell surface (Amsterdam, Netherlands)
影响因子：
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作者：
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通讯作者：
Vollmer W
DOI：
10.1073/pnas.1400376111
发表时间：
2014-06-03
影响因子：
11.1
作者：
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通讯作者：
Simorre, Jean-Pierre
DOI：
10.1107/s2053230x19004011
发表时间：
2019-05-01
影响因子：
0.9
作者：
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通讯作者：
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