Desynchronization of multivesicular release enhances Purkinje cell output.
Desynchronization of multivesicular release enhances Purkinje cell output.
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多囊泡释放的去同步增强了浦肯野细胞的输出。
DOI：
10.1016/j.neuron.2011.03.029
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发表时间：
2011
期刊：
影响因子：
16.2
通讯作者：
Wadiche,JacquesI
中科院分区：
文献类型：
--
作者：
Rudolph,Stephanie;Overstreet-Wadiche,Linda;Wadiche,JacquesI
动作电位后神经递质填充囊泡的释放以离散的时间过程发生：亚毫秒阶段性释放，可以通过活动进行去激活，然后是持续数十毫秒的“延迟释放”。延迟释放在突触整合中有一个明确的作用，但尚不清楚相位释放的去极化是否具有生理后果。在攀缘纤维与浦肯野细胞的突触中，多个囊泡的同步融合对于产生复杂的尖峰至关重要。在这里，我们表明，在生理频率的刺激驱动的时间分散的囊泡进行多泡释放，导致在毫秒时间尺度上的EPSC减慢。值得注意的是，EPSC动力学的这些变化强烈地改变浦肯野细胞复合体穗的方式，促进轴突繁殖的个人小穗。因此，多泡释放的去极化增强了复杂尖峰的精确和有效的信息传递。
The release of neurotransmitter-filled vesicles after action potentials occurs with discrete time courses: submillisecond phasic release that can be desynchronized by activity followed by "delayed release" that persists for tens of milliseconds. Delayed release has a well-established role in synaptic integration, but it is not clear whether desynchronization of phasic release has physiological consequences. At the climbing fiber to Purkinje cell synapse, the synchronous fusion of multiple vesicles is critical for generating complex spikes. Here we show that stimulation at physiological frequencies drives the temporal dispersion of vesicles undergoing multivesicular release, resulting in a slowing of the EPSC on the millisecond timescale. Remarkably, these changes in EPSC kinetics robustly alter the Purkinje cell complex spike in a manner that promotes axonal propagation of individual spikelets. Thus, desynchronization of multivesicular release enhances the precise and efficient information transfer by complex spikes.