A draft genome assembly of the army worm, Spodoptera frugiperda
A draft genome assembly of the army worm, Spodoptera frugiperda
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DOI：
10.1016/j.ygeno.2014.06.005
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发表时间：
2014-08-01
期刊：
影响因子：
4.4
通讯作者：
Bhatnagar, Raj K.
中科院分区：
文献类型：
--
作者：
Kakumani, Pavan Kumar;Malhotra, Pawan;Bhatnagar, Raj K.
摘要夜蛾是一种重要的农业害虫，其基因组的缺失限制了对其生物学特性的研究。在本研究中，基因组DNA测序并组装成37，243个大小为358 Mb的支架，N50为53.7 kb。根据同一性程度，我们可以将锚305 Mb的基因组锚定到家蚕的所有28条染色体上。重复序列占总基因组的20.28%。此外，我们预测了11，595个基因，平均内含子长度为726 bp。对这些基因进行了结构域分析，结果表明Sf基因与B具有显著的同源性和表达模式。尽管KOG基因类别和某些蛋白质家族的代表性存在差异。目前对Sf基因组的研究将有助于表征细胞通路，以了解其生物学和鳞翅目家庭成员之间的比较进化研究，以帮助注释他们的基因组。(C)2014爱思唯尔公司All rights reserved.
Spodoptera is an agriculturally important pest insect and studies in understanding its biology have been limited by the unavailability of its genome. In the present study, the genomic DNA was sequenced and assembled into 37,243 scaffolds of size, 358 Mb with N50 of 53.7 kb. Based on degree of identity, we could anchor 305 Mb of the genome onto all the 28 chromosomes of Bombyx mori. Repeat elements were identified, which accounts for 20.28% of the total genome. Further, we predicted 11,595 genes, with an average intron length of 726 bp. The genes were annotated and domain analysis revealed that Sf genes share a significant homology and expression pattern with B. mori, despite differences in KOG gene categories and representation of certain protein families. The present study on Sf genome would help in the characterization of cellular pathways to understand its biology and comparative evolutionary studies among lepidopteran family members to help annotate their genomes. (C) 2014 Elsevier Inc. All rights reserved.