Expression and Functional Roles of the Pepper Pathogen-Induced bZIP Transcription Factor CabZIP2 in Enhanced Disease Resistance to Bacterial Pathogen Infection
Expression and Functional Roles of the Pepper Pathogen-Induced bZIP Transcription Factor CabZIP2 in Enhanced Disease Resistance to Bacterial Pathogen Infection
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DOI：
10.1094/mpmi-10-14-0313-r
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发表时间：
2015-07-01
影响因子：
3.5
通讯作者：
Lee, Sung Chul
中科院分区：
文献类型：
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作者：
Lim, Chae Woo;Baek, Woonhee;Lee, Sung Chul
从侵染野油菜黄单胞菌(Xanthomonas Campestris PV)的辣椒叶片中分离到一个辣椒bZIP转录因子基因CabZIP2。口水症。对CabZIP2-GFP融合蛋白在烟草中的瞬时表达分析表明，CabZIP2蛋白定位于细胞质和细胞核中。在酵母菌中，CabZIP2 N-末端的酸性结构域与GAL4 DNA结合域的融合是激活报告基因转录所必需的。在接种野油菜致病或致病菌株的辣椒植株中，诱导了CabZIP2的转录。口水症。水杨酸、茉莉酸甲酯和乙烯等与防御相关的激素也诱导了CabZIP2基因的表达。为了阐明CabZIP2基因在植物防御中的体内功能，采用了病毒诱导的辣椒基因沉默和拟南芥的过表达。CabZIP2基因沉默的辣椒植株对野生型野油菜致病菌株的侵染十分敏感。伴随着CaBPR1和CaAMP1等防御相关基因表达的减少。在转基因拟南芥中过表达CabZIP2可以增强对紫丁香假单胞菌的抗性。番茄DC3000。综上所述，这些结果表明CabZIP2参与了细菌抗病。
A pepper bZIP transcription factor gene, CabZIP2, was isolated from pepper leaves infected with a virulent strain of Xanthomonas campestris pv. vesicatoria. Transient expression analysis of the CabZIP2-GFP fusion protein in Nicotiana benthamiana revealed that the CabZIP2 protein is localized in the cytoplasm as well as the nucleus. The acidic domain in the N-terminal region of CabZIP2 that is fused to the GAL4 DNA-binding domain is required to activate the transcription of reporter genes in yeast. Transcription of CabZIP2 is induced in pepper plants inoculated with virulent or avirulent strains of X. campestris pv. vesicatoria. The CabZIP2 gene is also induced by defense-related hormones such as salicylic acid, methyl jasmonate, and ethylene. To elucidate the in vivo function of the CabZIP2 gene in plant defense, virus-induced gene silencing in pepper and overexpression in Arabidopsis were used. CabZIP2-silenced pepper plants were susceptible to infection by the virulent strain of X. campestris pv. vesicatoria, which was accompanied by reduced expression of defense-related genes such as CaBPR1 and CaAMP1. CabZIP2 overexpression in transgenic Arabidopsis plants conferred enhanced resistance to Pseudomonas syringae pv. tomato DC3000. Together, these results suggest that CabZIP2 is involved in bacterial disease resistance.