Evaluation of fecal samples from mares as a source of Rhodococcus equi for their foals by use of quantitative bacteriologic culture and colony immunoblot analyses
Evaluation of fecal samples from mares as a source of Rhodococcus equi for their foals by use of quantitative bacteriologic culture and colony immunoblot analyses
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DOI：
10.2460/ajvr.68.1.63
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发表时间：
2007-01-01
影响因子：
1
通讯作者：
Martens, Ronald J.
中科院分区：
文献类型：
--
作者：
Grimm, Michael B.;Cohen, Noah D.;Martens, Ronald J.
样本群体-来自肯塔基州一个农场的171匹马和171匹马驹（2004年和2005年期间进行了评价）。（分娩前2例，分娩后2例），采用定量细菌培养和菌落免疫印迹法，测定了马立克次体粪便标本中马立克次体总浓度和强毒力马立克次体浓度技术，分别。比较了发生R equi相关性肺炎的马驹的血药浓度和未受影响马驹的血药浓度。每年的数据分别进行了分析。结果-R equi-associated肺炎53 171（31%）马驹。每匹母马在至少1个时间点检测到强毒马蛔虫的粪便散毒;在所有时间点，171只母马中有62只（36%）的细菌学培养结果呈阳性。然而，与未受影响的马驹的母鼠相比，患有马雷氏杆菌相关性肺炎的马驹的母鼠中总的或毒性马雷氏杆菌的粪便浓度没有显著差异。结论和临床相关性结果表明，患有马雷氏杆菌相关性肺炎的马驹的母鼠在粪便中没有比未受影响的马驹的母鼠排出更多的马雷氏杆菌;因此，马驹的马立克次体感染与其母体的粪便排出量相对较大无关。然而，在至少I个时间点期间，在所有猪的粪便中检测到毒性马呼肠杆菌，这表明猪可能是周围环境中马呼肠杆菌的重要来源。
Objective-To determine whether mares are a clinically important source of Rhodococcus equi for their foals.Sample Population-171 mares and 171 foals from a farm in Kentucky (evaluated during 2004 and 2005).Procedures-At 4 time points (2 before and 2 after parturition), the total concentration of R equi and concentration of virulent R equi were determined in fecal specimens from mares by use of quantitative bacteriologic culture and a colony immunoblot technique, respectively. These concentrations for mares of foals that developed R equi-associated pneumonia and for mares with unaffected foals were compared. Data for each year were analyzed separately.Results-R equi-associated pneumonia developed in 53 of 171 (31%) foals. Fecal shedding of virulent R equi was detected in at least 1 time point for every mare; bacteriologic culture results were positive for 62 of 171 (36%) mares at all time points. However, compared with dams of unaffected foals, fecal concentrations of total or virulent R equi in dams of foals with R equi-associated pneumonia were not significantly different.Conclusions and Clinical Relevance-Results indicate that dams of foals with R equi-associated pneumonia did not shed more R equi in feces than dams of unaffected foals; therefore, R equi infection in foals was not associated with comparatively greater fecal shedding by their dams. However, detection of virulent R equi in the feces of all mares during at least I time point suggests that mares can be an important source of R equi for the surrounding environment.