Utility of the mitochondrial cytochrome oxidase II gene for resolving relationships among black flies (Diptera: simuliidae).
Utility of the mitochondrial cytochrome oxidase II gene for resolving relationships among black flies (Diptera: simuliidae).
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线粒体细胞色素氧化酶 II 基因在解决黑蝇（双翅目：拟蝇科）之间的关系中的用途。
DOI：
10.1006/mpev.2000.0807
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Powers,TO
中科院分区：
文献类型：
--
作者：
Pruess,KP;Adams,BJ;Parsons,TJ;Zhu,X;Powers,TO
完整的线粒体细胞色素氧化酶II基因测序17黑蝇，代表13个推定的物种，并用于推断系统发育关系。一种蠓（Paratanytarsus sp.）三种蚊子（埃及伊蚊、四斑按蚊和致倦库蚊）被用作外群分类群。所有外群类群都与黑蝇高度不同。基于加权简约性（先验和后验），最大似然和相邻连接（对数决定距离）的系统发育树在拓扑上不同，更深的节点是最不支持的。所有的分析都支持目前的物种群分类，但这些群体之间的关系没有得到很好的解决。大部分的系统发育信号来自密切相关的姐妹类群。CO-II基因可能是有用的探索或低于亚属水平的关系，但在更高的分类学水平的价值值得怀疑。加权方法得到的系统发育结果类似于其他作者报道的其他昆虫CO-II数据集。基于CO-II基因的系统发育关系的最佳估计，并讨论了与目前的黑蝇分类。
The complete mitochondrial cytochrome oxidase II gene was sequenced from 17 black flies, representing 13 putative species, and used to infer phylogenetic relationships. A midge (Paratanytarsus sp.) and three mosquitoes (Aedes aegypti, Anopheles quadrimaculatus, and Culex quinquefasciatus) were used as outgroup taxa. All outgroup taxa were highly divergent from black flies. Phylogenetic trees based on weighted parsimony (a priori and a posteriori), maximum likelihood, and neighbor-joining (log-determinant distances) differed topologically, with deeper nodes being the least well-supported. All analyses supported current classification into species groups but relationships among those groups were poorly resolved. The majority of phylogenetic signal came from closely related sister taxa. The CO-II gene may be useful for exploring relationships at or below the subgeneric level, but is of questionable value at higher taxonomic levels. The weighting method employed gave phylogenetic results similar to those reported by other authors for other insect CO-II data sets. A best estimate of phylogenetic relationships based on the CO-II gene is presented and discussed in relation to current black fly classification.