A Near-infrared Fluorogenic Probe with Fast Response for Detecting Sodium Dithionite in Living Cells
A Near-infrared Fluorogenic Probe with Fast Response for Detecting Sodium Dithionite in Living Cells
复制标题
一种快速响应的近红外荧光探针，用于检测活细胞中的连二亚硫酸钠
DOI：
10.1016/j.saa.2020.118887
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Ronghua Yang
中科院分区：
文献类型：
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作者：
Xizi Yu;Lie Xiang;Sheng Yang;Shuanglin Qu;Xianqing Zeng;Yibo Zhou;Ronghua Yang
开发可靠的荧光探针对于准确监测生物系统中的连二亚硫酸钠（Na 2S 2 O 4，SDT）至关重要，但目前报道的偶氮荧光探针存在激发/发射波长短和响应速度相对较慢的问题。为了解决这一问题，本文提出了一种新的近红外发射荧光探针，命名为DCM-MQ，由一个二氰基亚甲基苯并吡喃荧光报告分子和一个1-甲基喹啉作为识别部分组成。基于DCM-MQ的特异性还原机理，DCM-MQ对SDT具有快速的比色和荧光识别（小于3 s）、较大的斯托克斯位移（112 nm）和较高的灵敏度（检测限为19 nM）。荧光成像结果表明，DCM-MQ可用于活体SDT的监测。
Developing a reliable fluorescence probe is crucial for accurately monitoring sodium dithionite (Na2S2O4, SDT) in biosystems, but the current reported azo-based ones suffers from short excitation/emission wavelengths and relative slow response speed. To address this issue, we herein present a novel near-infrared emissive fluorescence probe for SDT, namelyDCM-MQ, consisting of a dicyanomethylene-benzopyran fluorogenic reporter and a 1-methylquinolinium as recognition moiety. On the basis of the specific reduction mechanism,DCM-MQexhibited a rapid colorimetric and fluorescent recognition for SDT (less than 3 s) with large Stokes shift (112 nm) and high sensitivity (detection limit was 19 nM). The fluorescence imaging results demonstrate thatDCM-MQis competent for monitoring SDT in living systems.