Blinded, multicenter comparison of methods to detect a drug-resistant mutant of human immunodeficiency virus type 1 at low frequency
Blinded, multicenter comparison of methods to detect a drug-resistant mutant of human immunodeficiency virus type 1 at low frequency
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DOI：
10.1128/jcm.00449-06
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发表时间：
2006-07-01
影响因子：
9.4
通讯作者：
Mellors, John W.
中科院分区：
文献类型：
--
作者：
Halvas, Elias K.;Aldrovandi, Grace M.;Mellors, John W.
我们使用含有突变型-野生型混合物的盲法测试小组，测定了10种技术检测和量化人类免疫缺陷病毒1型常见耐药突变体（逆转录酶密码子103上的赖氨酸到天门汀）的能力，突变型的突变体范围为0.01%至100%。两种技术，等位基因特异性逆转录酶PCR和Ty1HRT酵母系统，可以将突变量化到0.1 - 0.4%。这些技术应有助于确定低频耐药突变体对抗逆转录病毒治疗反应的影响。
We determined the abilities of 10 technologies to detect and quantify a common drug-resistant mutant of human immunodeficiency virus type 1 (lysine to asparagine at codon 103 of the reverse transcriptase) using a blinded test panel containing mutant-wild-type mixtures ranging from 0.01% to 100% mutant. Two technologies, allele-specific reverse transcriptase PCR and a Ty1HRT yeast system, could quantify the mutant down to 0.1 to 0.4%. These technologies should help define the impact of low-frequency drug-resistant mutants on response to antiretroviral therapy.