Age related changes in the tunica media of the vertebral artery: implications for the assessment of vessels injured by trauma
Age related changes in the tunica media of the vertebral artery: implications for the assessment of vessels injured by trauma
复制标题
DOI：
10.1136/jcp.54.2.139
复制
发表时间：
2001-02-01
影响因子：
3.4
通讯作者：
Burns, J
中科院分区：
文献类型：
--
作者：
Johnson, CP;Baugh, R;Burns, J
AIMS-为了提供一个说明，详细的半定量分析的重要退行性变化沿着长度的椎动脉，使病理学家面临着调查一个致命的动脉损伤可以识别重要的预先存在的壁abnormalities. Methods-10横向环采取沿着34椎动脉从17个科目和染色切片准备使用苏木精和伊红和苦天狼星红的方法。常规显微镜检查后，使用目镜分划板定量图尼卡中膜中的弹性纤维、胶原和平滑肌细胞核。估计的严重程度和程度的弹性组织碎片，胶原瘢痕，内膜增厚/动脉粥样硬化，然后undertaked.Results-Smooth肌肉计数保持恒定沿着动脉，但胶原计数较高，弹性计数大幅降低颅内段内。弹性纤维断裂在婴儿期就被发现，并在成年早期中度进展，但在以后的生活中出现了相当大的胶原性瘢痕。有些人在35岁之前表现出严重的碎片和疤痕。退行性改变通常是局灶性的，几乎完全保留了颅内段。动脉粥样硬化随着年龄的增长而增加，但很少严重，并且在40岁以上似乎没有明显恶化。一个不寻常的安排的胶原组织内的上颈loops.Conclusion-Damaged椎动脉需要广泛采样，以允许适当的组织学评估。苦味酸天狼星红法成功地描绘了壁的精细结缔组织结构和早期退行性变化。对年龄和部位特异性变化的了解应使病理学家更容易识别重要的预先存在的异常。
Aims-To provide an illustrated, detailed semiquantitative analysis of the important degenerative changes along the length of the vertebral artery so that pathologists faced with investigating a fatal arterial injury can identify important pre-existing wall abnormalities.Methods-Ten transverse annuli were taken along 34 vertebral arteries from 17 subjects and stained sections were prepared using haematoxylin and eosin and the picro-sirius red method. After routine microscopy, the elastic fibres, collagen, and smooth muscle nuclei in the tunica media were quantified using an eyepiece graticule. An estimate of the severity and extent of elastic tissue fragmentation, collagenous scarring, and intimal thickening/atheroma was then undertaken.Results-Smooth muscle counts remained constant along the artery but collagen counts were higher and elastic counts substantially lower within the intracranial segment. Elastic fibre fragmentation was recognised in infancy and was moderately advanced by early adulthood but considerable collagenous scarring developed later in life. Some individuals demonstrated severe fragmentation and scarring before the age of 35 years. The degenerative changes were often focal and spared the intracranial segment almost completely. Atheroma increased with age but was rarely severe and appeared not to worsen appreciably beyond the age of 40 years. An unusual arrangement of the collagenous tissue was described within the upper cervical loops.Conclusion-Damaged vertebral arteries need to be sampled extensively to allow a proper histological assessment. The picro-sirius red method was successful in delineating the fine connective tissue structure of the wall and early degenerative changes. An understanding of the age and site specific changes should allow the pathologist to recognise important preexisting abnormalities more easily.