DISSEMINATED CYTOMEGALO-VIRUS INFECTION - MOLECULAR ANALYSIS OF VIRUS AND LEUKOCYTE INTERACTIONS IN VIREMIA
DISSEMINATED CYTOMEGALO-VIRUS INFECTION - MOLECULAR ANALYSIS OF VIRUS AND LEUKOCYTE INTERACTIONS IN VIREMIA
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DOI：
10.1172/jci113313
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发表时间：
1988-01-01
影响因子：
15.9
通讯作者：
JORDAN, MC
中科院分区：
文献类型：
--
作者：
SALTZMAN, RL;QUIRK, MR;JORDAN, MC
病毒血症是播散性巨细胞病毒（CMV）感染和疾病的标志。利用传统的病毒培养和亚基因组克隆的CMV DNA探针检测白细胞内的病毒DNA，我们研究了病毒血症性CMV感染免疫功能低下患者的病毒-细胞相互作用。在17/17个富含多形核白细胞的样本中，通过培养恢复了巨细胞病毒。16/17（94%）采用斑点杂交检测病毒DNA。相比之下，单核细胞富集的样本在培养中仅7/15（47%）产生感染性巨细胞病毒；尽管如此，在16/17的检测样本中存在病毒DNA。多形核细胞中CMV DNA的数量明显大于单核白细胞（平均每100个细胞13.1 vs. 9.1估计病毒基因组当量），并且无论存在多少病毒DNA， CMV总是从这些细胞中恢复。然而，当同时收集的粒细胞和单核细胞中的CMV DNA数量几乎相同（分别为6.3和7.1基因组当量）时，无法从单核细胞中恢复感染性CMV。尽管可能有几种解释，但这些数据都与CMV在病毒血症期间以成熟感染形式存在于粒细胞中的观点一致。病毒与单核细胞的相互作用似乎更为复杂，特别是在那些含有巨细胞病毒DNA但不产生感染性病毒的细胞中。
Viremia is a hallmark of disseminated cytomegalovirus (CMV) infectin and disease. Using conventional virus culture and a subgenomic cloned CMV DNA probe to detect viral DNA within leukocytes, we studied the virus-cell interactions involved in immunocompromised patients with viremic CMV infection. CMV was recovered by culture in 17/17 samples enriched for polymorphonuclear leukocytes. Viral DNA was detected by dot-blot hybridization in 16/17 (94%). In contrast, samples enriched for mononuclear cells yielded infectious CMV in culture in only 7/15 (47%) instances; nonetheless, viral DNA was present in 16/17 samples probed. The quantity of CMV DNA in polymorphonuclear cells was significantly greater than in mononuclear leukocytes (mean 13.1 vs. 9.1 estimated viral genome equivalents per 100 cells, respectively), and CMV was always recovered from thse cells regardless of the amount of viral DNA present. Yet, when the amounts of CMV DNA were virtually identical in granulocytes and mononuclear cells (6.3 and 7.1 genomic equivalents, respectively) collected simultaneously, infectious CMV could not be recovered from mononuclear cells. Although several interpretations are possible, these data are consistent with the view that CMV exists within granulocytes in a mature infectious form during viremia. The virus interactions with mononuclear cells appear to be more complex, particularly in those cells that contain CMV DNA but do not yield infectious virus.