Characterization of chlorinated valepotriates from Valeriana jatamansi
Characterization of chlorinated valepotriates from Valeriana jatamansi
复制标题
DOI：
10.1016/j.phytochem.2012.08.015
复制
发表时间：
2013-01-01
期刊：
影响因子：
3.8
通讯作者：
Zhang, Wei-dong
中科院分区：
文献类型：
--
作者：
Lin, Sheng;Zhang, Zhong-Xiao;Zhang, Wei-dong
采用高效液相色谱- pda - ms和薄层色谱分析方法，从全株缬草（Valeriana wallichii DC.）中寻找少量的细胞毒性氯代戊酸酯。结果分离出15种氯化戊酸酯，命名为氯戊酸A-O，以及6种已知的类似物，(15,3r,5R,7S,8S,9S)-3,8-环氧-1,5-二羟基戊氯，戊酸B，氯戊酸B，石榴素B， (15,3r,5R,7S,8S,95)-3,8-环氧-1- o -乙基-5-羟基戊氯，和(1R,3R,5R,7S,8S,9S)-3,8-环氧-1- o -乙基-5-羟基戊氯。通过二维核磁共振实验和同核和异核对其结构进行了分析。氯戊酸盐K-N、氯戊酸盐和rupesin B对肺腺癌（a549）、转移性前列腺癌（PC-3M）、结肠癌（HCT-8）和肝癌（Bel 7402）的IC50值为0.89-9.76 μ M. (C) 2012 Elsevier Ltd.。版权所有。
HPLC-PDA-MS and TLC analysis were used to look for minor cytotoxic chlorinated valepotriates from whole plants of Valeriana jatamansi (syn. Valeriana wallichii DC.). This resulted in isolation of 15 chlorinated valepotriates, designated as chlorovaltrates A-O, together with six known analogues, (15,3R,5R,7S,8S,9S)-3,8-epoxy-1,5-dihydroxyvalechlorine, volvaltrate B, chlorovaltrate, rupesin B, (15,3R,5R,7S,8S,95)-3,8-epoxy-1-O-ethyl-5-hydroxyvalechlorine, and (1R,3R,5R,7S,8S,9S)-3,8-epoxy-1-O-ethyl-5-hydroxyvalechlorine. Their structures were elucidated by spectroscopic methods including homo- and heteronuclear two-dimensional NMR experiments. Chlorovaltrates K-N, chlorovaltrate and rupesin B showed moderate cytotoxicity against lung adenocarcinoma (A 549), metastatic prostate cancer (PC-3M), colon cancer (HCT-8) and hepatoma (Bel 7402) cell lines with IC50 values of 0.89-9.76 mu M. (C) 2012 Elsevier Ltd. All rights reserved.