Epidermal growth factor receptor and transforming growth factor-β signaling contributes to variation for wing shape in Drosophila melanogaster
Epidermal growth factor receptor and transforming growth factor-β signaling contributes to variation for wing shape in Drosophila melanogaster
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DOI：
10.1534/genetics.105.053868
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发表时间：
2006-07-01
期刊：
影响因子：
3.3
通讯作者：
Gibson, Greg
中科院分区：
文献类型：
--
作者：
Dworkin, Ian;Gibson, Greg
果蝇翅膀的发育是研究遗传网络及其在发育过程中作用的一个常用模型系统。特别是，RTK和TGF-β调节网络似乎涉及翅膀发育的许多方面，包括发育中的成虫翅盘的图案化、细胞决定、生长、增殖和存活。然而，很少有人知道如何微妙的变化，这些基因的功能可能有助于数量变化的翅膀形状，本身。在这项研究中，50个插入突变，代表RTK，刺猬，TGF-β途径中的43个位点，以及它们的遗传相互作用因子，用于研究这些网络对翅膀形状的作用。为了同时检查遗传背景如何调节突变的影响，将每个插入渗入到两个野生型遗传背景中。使用几何形态测量方法，结果表明，当作为杂合体测量时，这些突变中的大多数对翅膀的形状有深远的影响，但对翅膀的大小没有影响。为了研究每个突变如何影响翅膀形状之间的关系，使用了层次聚类。与以前观察到的环境渠化，这些突变一般不会增加行内变异相对于野生型。这些结果提供了一个切入点到遗传学的翅膀形状和复杂的表型解剖的框架内进行了讨论。
Wing development in Drosophila is a common model system for the dissection of genetic networks and their roles during development. In particular, the RTK and TGF-beta regulatory networks appear to be involved with numerous aspects of wing development, including patterning, cell determination, growth, proliferation, and survival in the developing imaginal wing disc. However, little is known as to how subtle changes in the function of these genes may contribute to quantitative variation for wing shape, per se. In this study 50 insertional mutations, representing 43 loci in the RTK, Hedgehog, TGF-beta pathways, and their genetically interacting factors were used to study the role of these networks on wing shape. To concurrently examine how genetic background modulates the effects of the mutation, each insertion was introgressed into two wild-type genetic backgrounds. Using geometric morphometric methods, it is shown that the majority of these mutations have profound effects on shape but not size of the wing when measured as heterozygotes. To examine the relationships between how each mutation affects wing shape hierarchical clustering was used. Unlike previous observations of environmental canalization, these mutations did not generally increase within-line variation relative to their wild-type counterparts. These results provide an entry point into the genetics of wing shape and are discussed within the framework of the dissection of complex phenotypes.