Immune responses and protection against foot-and-mouth disease virus (FMDV) challenge in swine vaccinated with adenovirus-FMDV constructs
Immune responses and protection against foot-and-mouth disease virus (FMDV) challenge in swine vaccinated with adenovirus-FMDV constructs
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DOI：
10.1016/s0264-410x(00)00384-4
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发表时间：
2001-02-28
期刊：
影响因子：
5.5
通讯作者：
Grubman, MJ
中科院分区：
文献类型：
--
作者：
Mayr, GA;O'Donnell, V;Grubman, MJ
将编码口蹄疫病毒（FMDV）衣壳蛋白和3C蛋白酶编码区的复制缺陷型腺病毒5型（Ad 5-FMDV 3CWT）用于免疫猪。测试了利用1 × 10（8）空斑形成单位（pfu）的单次接种或接种1 × 10（8）随后加强5 × 10（8）pfu的Ad 5-FMDV 3 CWT，沿着接种并加强使用编码FMDV衣壳编码区的腺病毒和3C蛋白酶的失活形式（Ad 5-FMDV 3 CMUT）。使用免疫沉淀、中和和IgM特异性ELISA测定，检查从这些动物收集的血清中是否存在FMDV特异性抗体。IgG 1和IgG 2通过将接种疫苗的猪与FMDV感染的猪接触并监测疾病体征和血清抗体水平的变化来进行有效性研究。Ad5-FMDV 3CMUT。其不能产生FMDV衣壳结构，不能引发FMDV中和抗体或保护免受FMD。单次接种Ad 5-FMDV 3CWT可产生FMDV特异性中和抗体，并减少攻毒猪的临床症状，但未能完全保护大多数猪免受FMD的侵害。接受Ad 5-FMDV 3CWT初次接种，随后在4周时加强接种的猪产生高水平的FMDV中和抗体，导致6头猪中的5头得到完全保护，其余动物的疾病有限。两次给药方案的疗效增加与FMDV特异性IgG 1和IgG 2抗体水平升高相关。出版社：Elsevier Science Ltd
A replication-defective adenovirus 5 encoding foot-and-mouth disease virus (FMDV) capsid and 3C proteinase coding regions (Ad5-FMDV3CWT) was used to vaccinate swine. A single inoculation utilizing 1 x 10(8) plaque forming units (pfu) or an inoculation of 1 x 10(8) followed by a boost of 5 x 10(8) pfu Ad5-FMDV3CWT were tested, along with an inoculation and boost using an adenovirus encoding the FMDV capsid coding region and an inactive form of the 3C proteinase (Ad5-FMDV3CMUT). Sera collected from these animals were examined for the presence of FMDV-specific antibodies using immunoprecipitation, neutralization, and ELISA assays specific for IgM. IgG1 and IgG2. Efficacy studios were performed by placing the vaccinated swine in contact with an FMDV-infected swine and monitoring for signs of disease and changes in serum antibody levels. Ad5-FMDV3CMUT. which is unable to produce FMDV capsid structures, did not elicit FMDV-neutralizing antibodies or protect against FMD. Single inoculation with Ad5-FMDV3CWT generated FMDV-specific neutralizing antibodies, and reduced clinical signs in challenged swine, but failed to completely protect the majority of swine from FMD. Swine which received a primary vaccination with Ad5-FMDV3CWT followed by the boost at 4 weeks generated high levels of FMDV-neutralizing antibodies resulting in complete protection of five of the six swine and limited disease in the remaining animal. Increased efficacy of the two-dose regimen was associated with heightened levels of FMDV-specific IgG1 and IgG2 antibodies. Published by Elsevier Science Ltd.