Graphene Quantum Dots-based Electrochemical Biosensor for Catecholamine Neurotransmitters Detection
Graphene Quantum Dots-based Electrochemical Biosensor for Catecholamine Neurotransmitters Detection
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DOI：
10.1002/elan.201700825
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发表时间：
2018-08-01
期刊：
影响因子：
3
通讯作者：
Cabaj, Joanna
中科院分区：
文献类型：
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作者：
Baluta, Sylwia;Lesiak, Anna;Cabaj, Joanna
基于石墨烯量子点（GQD）和漆酶修饰的玻碳电极（GC），开发了一种有用的电化学传感方法，用于肾上腺素（EP）检测。通过将漆酶固定在涂有碳纳米粒子的电极的电活性层中，设计并构建了微型GC生物传感器。这种传感装置利用 EP 催化氧化为肾上腺素醌。检测过程基于儿茶酚胺在漆酶存在下的氧化。在优化的条件下，分析性能表现出较高的灵敏度-2.9AmM(-1)cm(-2)、较宽的线性范围(1-120x10(-6)M)选择性、检测限为83nM。此外，该方法还成功应用于标记药理样品中的EP测定。
An useful electrochemical sensing approach was developed for epinephrine (EP) detection based on graphene quantum dots (GQDs) and laccase modified glassy carbon electrodes (GC). The miniature GC biosensor was designed and constructed via the immobilization of laccase in an electroactive layer of the electrode coated with carbon nanoparticles. This sensing arrangement utilized the catalytic oxidation of EP to epinephrine quinone. The detection process was based on the oxidation of catecholamine in the presence of the enzyme - laccase. With the optimized conditions, the analytical performance demonstrated a high degree of sensitivity -2.9AmM(-1)cm(-2), selectivity in a broad linear range (1-120x10(-6)M) with detection limit of 83nM. Moreover, the method was successfully applied for EP determination in labeled pharmacological samples.