ISOLATION OF VIRUS CAUSING HEMORRHAGIC-FEVER WITH RENAL SYNDROME (HFRS) THROUGH A CELL-CULTURE SYSTEM
ISOLATION OF VIRUS CAUSING HEMORRHAGIC-FEVER WITH RENAL SYNDROME (HFRS) THROUGH A CELL-CULTURE SYSTEM
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DOI：
10.7883/yoken1952.36.17
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发表时间：
1983-01-01
期刊：
影响因子：
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通讯作者：
URASAWA, S
中科院分区：
文献类型：
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作者：
KITAMURA, T;MORITA, C;URASAWA, S
将3家医疗机构肾综合征出血热(HFRS)暴发期间采集的23份大鼠肺标本接种于非洲绿猴肾Vero-E6细胞单层。经过几次盲传后，从其中两个样本中分离出一种在细胞培养中连续生长并与已知的HFRS阳性血清发生特异性反应的试剂。这两个分离株在抗原性上完全相同。根据其与E6细胞适应株、汉滩76-118株的抗原同源性以及与不同病例血清的特异性反应，确定该毒株为引起肾综合征出血热的病毒。
Twenty-three rat lung specimens collected in outbreaks of hemorrhagic fever with renal syndrome (HFRS) in 3 medical institutions were inoculated onto the African green monkey kidney Vero-E6 cell monolayers. After several blind passages, an agent growing serially in the cell cultures and reacting specifically with known HFRS-positive sera was isolated from 2 of these specimens. The 2 isolates were antigenically identical to each other. The agent, named strain SR-11, was identified as the causative virus of HFRS by its antigenic identity with E6 cell-adapted HFRS virus, Hantaan 76-118 strain and the specific reactions with sera from various HFRS cases.