Ginkgo biloba extract EGb 761 and Wisconsin ginseng delay sarcopenia in Caenorhabditis elegans
Ginkgo biloba extract EGb 761 and Wisconsin ginseng delay sarcopenia in Caenorhabditis elegans
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DOI：
10.1093/gerona/62.12.1337
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发表时间：
2007-12-01
影响因子：
5.1
通讯作者：
Luo, Yuan
中科院分区：
文献类型：
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作者：
Cao, Zhiming;Wu, YanJue;Luo, Yuan
以前，我们报道，标准化银杏叶提取物EGb 761延长寿命，增加秀丽隐杆线虫的抗应激能力。在这项研究中，确定了年龄依赖性肌肉变性或肌肉减少症的药理学调节。转基因长用EGb 761或威斯康星州人参喂养表达定位于体壁肌肉和外阴肌肉核中的绿色荧光蛋白（GFP）-MYO-3的线虫品系（PD 4251），并通过定量GFP荧光分析肌肉完整性。EGb 761和威斯康星州人参均显著延迟肌肉减少症。人参是更有效的蠕虫更先进的年龄，这是一致的透射电子显微镜观察到的超微结构变化。此外，这两种药物改善年龄相关的运动行为，包括运动，身体弯曲，和咽泵的下降。这些结果表明，药理学延长寿命是维持组织功能能力的结果，C。Elegans是一种有效的模型系统，用于测试延迟肌肉减少症进展的治疗干预。
Previously we reported that the standardized Ginkgo biloba extract EGb 761 extended life span and increased stress resistance in Caenorhabditis elegans. In this study, pharmacological modulation of age-dependent muscle degeneration, or sarcopenia, was determined. Transgenic C. elegans strain (PD4251) expressing green fluorescent protein (GFP)-MYO-3, localized in body wall muscles and vulval muscle nuclei, were fed with EGb 761 or Wisconsin Ginseng, and muscle integrity was analyzed by quantification of GFP fluorescence. Both EGb 761 and Wisconsin Ginseng significantly delayed sarcopenia. Ginseng was more effective in worms of more advanced age, which is consistent with the ultrastructural changes observed by transmission electron microscopy. Furthermore, both agents ameliorated age-associated decline of locomotive behaviors including locomotion, body bend, and pharyngeal pumping. These results suggest that pharmacological extension of life span is a consequence of maintaining functional capacity of the tissue, and that C. elegans is a valid model system for testing therapeutic intervention for delaying the progress of sarcopenia.