Nuclear pores dilate and constrict in cellulo
Nuclear pores dilate and constrict in cellulo
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DOI：
10.1126/science.abd9776
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发表时间：
2021-12-10
期刊：
影响因子：
56.9
通讯作者：
Beck, Martin
中科院分区：
文献类型：
--
作者：
Zimmerli, Christian E.;Allegretti, Matteo;Beck, Martin
在真核细胞中，核孔复合物（nuclear pore complex，NPCs）融合核内外膜并介导核质交换。它们由30种不同的核孔蛋白组成，并在水中央通道周围形成圆柱形结构。这种建筑在空间和时间上是高度动态的。已经报道了NPC直径的变化，但生理情况和分子细节仍然未知。在这里，我们结合冷冻电子断层扫描与综合结构建模，以捕捉细胞中相应的大规模构象变化的分子电影。虽然按指数增长的细胞的NPC采取了扩张的构象，他们可逆地收缩后，细胞能量耗尽或高渗渗透应激的条件。我们的数据指向一个模型，其中核膜张力与NPC的构象有关。
In eukaryotic cells, nuclear pore complexes (NPCs) fuse the inner and outer nuclear membranes and mediate nucleocytoplasmic exchange. They are made of 30 different nucleoporins and form a cylindrical architecture around an aqueous central channel. This architecture is highly dynamic in space and time. Variations in NPC diameter have been reported, but the physiological circumstances and the molecular details remain unknown. Here, we combined cryo-electron tomography with integrative structural modeling to capture a molecular movie of the respective large-scale conformational changes in cellulo. Although NPCs of exponentially growing cells adopted a dilated conformation, they reversibly constricted upon cellular energy depletion or conditions of hypertonic osmotic stress. Our data point to a model where the nuclear envelope membrane tension is linked to the conformation of the NPC.